Key Product Details

Species Reactivity

Chicken

Applications

Western Blot, ELISA, SDS-Page

Label

Unconjugated

Antibody Source

Chicken IgG

Format

Purified
View all IgG Fc Isotype Controls »

Product Specifications

Specificity

No reaction was observed against anti-Chicken IgG F(ab')2 or anti-Papain.

Host

Chicken

Isotype

IgG

Description

This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum, anti-Chicken IgG and anti-Chicken IgG F(c)

Store vial at 4C prior to opening. Stable at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.

Scientific Data Images for Chicken IgG Fc Isotype Control

SDS-PAGE: Chicken IgG Fc Isotype Control [NBP1-96786]

SDS-PAGE: Chicken IgG Fc Isotype Control [NBP1-96786]

SDS-Page: Chicken IgG Fc Isotype Control [NBP1-96786] - SDS-PAGE of Chicken IgG F(c) Fragment. Lane M: 5 uL Opal Prestained Marker. Lane 1: Reduced Chicken IgG Whole Molecule. Lane 2: Reduced Chicken IgG F(c) Fragment. Lane 3: Reduced Chicken IgG Fab Fragment. Lane 4: Reduced Chicken IgM Whole Molecule. Load: 1 ug per lane. Predicted/Observed size: IgG at 72 and 25 kDa; F(c) at 25 kDa; Fab at 25 kDa; IgM at 75 kDa. Observed F(c) Fragment migrates slightly higher. Other bands: Chicken IgG heavy chain alternative splicing variant at approximately 40 kDa in Lane 1.

Applications for Chicken IgG Fc Isotype Control

Application
Recommended Usage

Western Blot

1:100 - 1:2000
Application Notes
This product has been tested by SDS-PAGE and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

Purified

Preservative

0.01% Sodium Azide

Concentration

Please contact technical services for concentration.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Product Documents for Chicken IgG Fc Isotype Control

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Chicken IgG Fc Isotype Control

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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FAQs

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