Goat anti-Human IgG Heavy Chain Secondary Antibody (Pre-adsorbed)
Novus Biologicals | Catalog # NBP2-68475
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Frozen, ELISA
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Format
Pre-adsorbed
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Product Specifications
Immunogen
Human IgG
Specificity
Goat anti Human IgG gamma chain antibody recognizes human IgG and demonstrates <1% cross-reactivity with other human immunoglobulin classes or subclasses. The antibody recognizes the Fc region of IgG, and has been pre-absorbed against mouse immunoglobulins to reduce species cross-reactivity.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Goat anti-Human IgG Heavy Chain Secondary Antibody (Pre-adsorbed)
ELISA: Goat anti-Human IgG Heavy Chain Secondary Antibody (Pre-adsorbed) [NBP2-68475] -
ELISA: Goat anti-Human IgG Heavy Chain Secondary Antibody (Pre-adsorbed) [NBP2-68475] - Antigen down ELISA showing binding of Goat anti Human IgG gamma chain:HRP to immobilized human IgG1 @ 5μgml-1.Applications for Goat anti-Human IgG Heavy Chain Secondary Antibody (Pre-adsorbed)
Application
Recommended Usage
ELISA
1 ug/ml - 5 ug/ml
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS
Format
Pre-adsorbed
Preservative
0.09% Sodium Azide
Concentration
0.8 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IgG Heavy Chain
Alternate Names
Immunoglobulin G, ImmunoglobulinG
Additional IgG Heavy Chain Products
Product Documents for Goat anti-Human IgG Heavy Chain Secondary Antibody (Pre-adsorbed)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Goat anti-Human IgG Heavy Chain Secondary Antibody (Pre-adsorbed)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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