Rabbit IgG Isotype Control

Novus Biologicals | Catalog # NBP1-97041

Novus Biologicals
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Key Product Details

Species Reactivity

Rabbit

Applications

Immunohistochemistry, Western Blot, ELISA, SDS-Page

Label

Unconjugated

Antibody Source

Rabbit IgG

Format

Purified
View all IgG Isotype Controls »

Product Specifications

Host

Rabbit

Isotype

IgG

Description

This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG and anti-Rabbit Serum.

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Rabbit IgG Isotype Control

Western Blot: Rabbit IgG Isotype Control [NBP1-97041]

Western Blot: Rabbit IgG Isotype Control [NBP1-97041]

Western Blot: Rabbit IgG Isotype Control [NBP1-97041] - Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule (NBP2-36463). Lane 2: Rabbit IgG F(ab) Fragment (NBP1-97016). Lane 3: Rabbit IgG F(c) Fragment (NBP1-96784). Lane 4: Rabbit IgM Whole Molecule (NBP1-96968). Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Blocked in blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins. (NBP1-72732) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.
Western Blot: Rabbit IgG Isotype Control [NBP1-97041]

Western Blot: Rabbit IgG Isotype Control [NBP1-97041]

Western Blot: Rabbit IgG Isotype Control [NBP1-97041]
SDS-PAGE: Rabbit IgG Isotype Control [NBP1-97041]

SDS-PAGE: Rabbit IgG Isotype Control [NBP1-97041]

SDS-Page: Rabbit IgG Isotype Control [NBP1-97041] - Lane 1: Rabbit IgG - Non-Reduced. Lane 2: Rabbit IgG - Reduced. Load: 1.0 ug per lane. Predicted/Observed Size: Non-reduced - 130 kDa, Reduced - 55 and 28 kDa for Rabbit IgG.
SDS-PAGE: Rabbit IgG Isotype Control [NBP1-97041]

SDS-PAGE: Rabbit IgG Isotype Control [NBP1-97041]

SDS-Page: Rabbit IgG Isotype Control [NBP1-97041] - SDS-PAGE of Rabbit IgG Isotype control. Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rabbit IgG Isotype control. Lane 2: Reduced Rabbit IgG F(ab) Fragment. Lane 3: Reduced Rabbit IgG F(c) Fragment. Lane 4: Reduced Rabbit IgM Whole Molecule. Load: 1 ug for F(ab) and F(c); 1.2 ug for IgG and IgM. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.
SDS-PAGE: Rabbit IgG Isotype Control [NBP1-97041]

SDS-PAGE: Rabbit IgG Isotype Control [NBP1-97041]

SDS-Page: Rabbit IgG Isotype Control [NBP1-97041] - SDS-PAGE of Rabbit IgG Isotype control. Lane 1: Non-reduced Rabbit IgG Isotype control. Lane 2: 5uL OPAL Pre-stained Marker. Lane 3: Reduced Rabbit IgG Isotype control. Load: 1ug per lane. Predicted/Observed size: Non-reduced at 150-170 kDa, Reduced at 55, 25 kDa.

Applications for Rabbit IgG Isotype Control

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunohistochemistry

1:10 - 1:500

SDS-Page

1:100 - 1:2000

Western Blot

1:100 - 1:2000
Application Notes
This product has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 1.0 ml deionized water (or equivalent).

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

Purified

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IgG

IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Gene Symbol

IGHG1

Additional IgG Products

Product Documents for Rabbit IgG Isotype Control

Certificate of Analysis

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Download SDS

Product Specific Notices for Rabbit IgG Isotype Control

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Citations for Rabbit IgG Isotype Control

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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