IgG2a Antibody (RM219) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25952

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM219 expressed in HEK293

Format

Azide and BSA Free
View all IgG2A Primary Antibodies »

Product Specifications

Immunogen

Mouse IgG2a

Specificity

This antibody reacts to the Fc region of mouse IgG2a. No cross reactivity with mouse IgG1, IgG2b, IgG2c, IgG3, IgM, IgA, IgE, human IgG, or rat IgG.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IgG2a Antibody (RM219) - Azide and BSA Free

IgG2a Antibody (RM219) - Azide and BSA Free

Western Blot: IgG2a Antibody (RM219) - Azide and BSA Free [NBP3-25952] -

Western Blot: IgG2a Antibody (RM219) - Azide and BSA Free [NBP3-25952] - Western blot of nonreduced(-) and reduced(+) mouse IgG2a, using Clone RM219. This antibody reacts to nonreduced IgG2a (~150 kDa).
IgG2a Antibody (RM219) - Azide and BSA Free

ELISA: IgG2a Antibody (RM219) - Azide and BSA Free [NBP3-25952] -

ELISA: IgG2a Antibody (RM219) - Azide and BSA Free [NBP3-25952] - ELISA of mouse immunoglobulins shows RM219 reacts to the Fc region of mouse IgG2a; no cross reactivity with IgG1, IgG2b, IgG2c, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM219 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
IgG2a Antibody (RM219) - Azide and BSA Free

ELISA: IgG2a Antibody (RM219) - Azide and BSA Free [NBP3-25952] -

ELISA: IgG2a Antibody (RM219) - Azide and BSA Free [NBP3-25952] - A titer ELISA of mouse IgG2a. The plate was coated with different amount of mouse IgG2a. A serial dilution of RM219 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
IgG2a Antibody (RM219) - Azide and BSA Free

ELISA: IgG2a Antibody (RM219) - Azide and BSA Free [NBP3-25952] -

ELISA: IgG2a Antibody (RM219) - Azide and BSA Free [NBP3-25952] - ELISA of mouse immunoglobulins shows RM219 reacts to the Fc region of mouse IgG2a; no cross reactivity with IgG1, IgG2b, IgG2c, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM219 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
IgG2a Antibody (RM219) - Azide and BSA Free

ELISA: IgG2a Antibody (RM219) - Azide and BSA Free [NBP3-25952] -

ELISA: IgG2a Antibody (RM219) - Azide and BSA Free [NBP3-25952] - A titer ELISA of mouse IgG2a. The plate was coated with different amount of mouse IgG2a. A serial dilution of RM219 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

Applications for IgG2a Antibody (RM219) - Azide and BSA Free

Application
Recommended Usage

ELISA

0.005 ug/ml - 0.2 ug/ml

Western Blot

0.5 ug/ml - 2 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IgG2A

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G2A Fc

Additional IgG2A Products

Product Documents for IgG2a Antibody (RM219) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for IgG2a Antibody (RM219) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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