Rat anti-Mouse IgG2a Heavy Chain Secondary Antibody (LO-MG2a-7) (BSA Free)
Novus Biologicals | Catalog # NBP2-68435
Key Product Details
Species Reactivity
Mouse
Applications
ELISA
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG1 Clone # LO-MG2a-7
Format
BSA Free
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Product Specifications
Immunogen
Purified IgG from BALB/c mice
Specificity
Rat anti Mouse IgG2a-Biotin, clone LO-MG2a-7, recognizes the gamma 2a heavy chain of mouse immunoglobulin and does not cross-react with other murine immunoglobulin classes or subclasses. The antibody recognizes an allotypic determinant upon mouse IgG2a. It recognizes the IgHIa allotype (as expressed in Balb/c mice), but not the IgHIb allotype (as expressed in C57/BL mice).
Clonality
Monoclonal
Host
Rat
Isotype
IgG1
Applications
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
5 ug/ml as coating antibody for ELISA.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Product Documents
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
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- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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