IgG2b Antibody (RM108)

Novus Biologicals | Catalog # NBP2-62009

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM108 expressed in HEK293
View all IgG2B Primary Antibodies »

Product Specifications

Immunogen

Mouse IgG2b

Specificity

This antibody reacts to the Fc region of mouse IgG2b. No cross reactivity with mouse IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IgG2b Antibody (RM108)

Western Blot: IgG2b Antibody (RM108) [Unconjugated] [NBP2-62009] - Western blot of nonreduced(-) and reduced(+) mouse IgG2b, using NBP2-62009. This antibody reacts to nonreduced IgG2b (~150 kDa) much stronger than the reduced g2b form (~50 kDa).
IgG2b Antibody (RM108)

ELISA: IgG2b Antibody (RM108) - Azide and BSA Free [NBP2-62009] -

ELISA: IgG2b Antibody (RM108) - Azide and BSA Free [NBP2-62009] - A titer ELISA of mouse IgG2b. The plate was coated with different amounts of mouse IgG2b. A serial dilution of RM108 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
IgG2b Antibody (RM108)

ELISA: IgG2b Antibody (RM108) - Azide and BSA Free [NBP2-62009] -

ELISA: IgG2b Antibody (RM108) - Azide and BSA Free [NBP2-62009] - ELISA of mouse immunoglobulins shows RM108 reacts to the Fc region of mouse IgG2b; no cross reactivity with IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM108 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

Applications for IgG2b Antibody (RM108)

Application
Recommended Usage

ELISA

0.005 ug/ml - 0.2 ug/ml

Western Blot

0.1 ug/ml-0.5 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50% Glycerol/PBS, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IgG2B

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G2B Fc

Additional IgG2B Products

Product Documents for IgG2b Antibody (RM108)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for IgG2b Antibody (RM108)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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