IgG3 Antibody (RM218)

Novus Biologicals | Catalog # NBP2-62010

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM218 expressed in HEK293
View all IgG3 Primary Antibodies »

Product Specifications

Immunogen

Mouse IgG3

Specificity

This antibody reacts to the Fab region of mouse IgG3. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgM, IgA, IgE, human IgG, or rat IgG.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IgG3 Antibody (RM218)

IgG3 Antibody (RM218)

ELISA: IgG3 Antibody (RM218) - Azide and BSA Free [NBP2-62010] -

ELISA: IgG3 Antibody (RM218) - Azide and BSA Free [NBP2-62010] - ELISA of mouse immunoglobulins shows RM218 reacts to both mouse IgG3 kappa and IgG3 lambda; No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgM, IgA, IgE, human IgG, or rat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM218 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
IgG3 Antibody (RM218)

ELISA: IgG3 Antibody (RM218) - Azide and BSA Free [NBP2-62010] -

ELISA: IgG3 Antibody (RM218) - Azide and BSA Free [NBP2-62010] - A titer ELISA of mouse IgG3. The plate was coated with different amounts of mouse IgG3. A serial dilution of RM218 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

Applications for IgG3 Antibody (RM218)

Application
Recommended Usage

ELISA

0.005 ug/ml-0.2 ug/ml

Western Blot

0.1 ug/ml - 0.5 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50% Glycerol/PBS, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IgG3

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G3

Alternate Names

DKFZp686H11213, FLJ39988, FLJ40036, FLJ40253, FLJ40587, FLJ40789, FLJ40834, IgG3, immunoglobulin heavy constant gamma 3 (G3m marker), MGC45809

Gene Symbol

IGHG3

Additional IgG3 Products

Product Documents for IgG3 Antibody (RM218)

Certificate of Analysis

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Product Specific Notices for IgG3 Antibody (RM218)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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