IgM Antibody (RM109) [Biotin]

Novus Biologicals | Catalog # NBP2-62012B

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Mouse

Applications

ELISA

Label

Biotin

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM109 expressed in HEK293
View all IgM Primary Antibodies »

Product Specifications

Immunogen

Mouse IgM

Specificity

This antibody reacts to mouse IgM. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgG3, IgA, IgE, human IgG, rat IgG, or goat IgG.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IgM Antibody (RM109) [Biotin]

IgM Antibody (RM109) [Biotin]

ELISA: IgM Antibody (RM109) - Azide and BSA Free [NBP2-62012B] -

ELISA: IgM Antibody (RM109) - Azide and BSA Free [NBP2-62012B] - A titer ELISA of mouse IgM. The plate was coated with different amounts of mouse IgM. A serial dilution of RM109 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
IgM Antibody (RM109) [Biotin]

ELISA: IgM Antibody (RM109) - Azide and BSA Free [NBP2-62012B] -

ELISA: IgM Antibody (RM109) - Azide and BSA Free [NBP2-62012B] - ELISA of mouse immunoglobulins shows RM109 reacts to mouse IgM; no cross reactivity with IgG1, IgG2a, IgG2b, IgG3, IgA, IgE, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM109 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

Applications for IgM Antibody (RM109) [Biotin]

Application
Recommended Usage

ELISA

0.005 ug/ml - 0.2 ug/ml
Application Notes
This primary antibody can also be used as a secondary antibody.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 50% Glycerol, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IgM

IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to its large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to its high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences. IgM measurement yields information about the body's immediate resistance and response to infection as well as information related to specific diseases. Decreased levels are associated with immune deficiency states, hereditary deficiencies, and myeloma. Increased levels can be associated with Waldenstrom's macroglobulinemia, chronic infection and hepatocellular disease.

Long Name

Immunoglobulin M

Alternate Names

AGM1, DKFZp686I15196, DKFZp686I15212, FLJ00385, immunoglobulin heavy constant mu, MGC104996, MGC52291, MU, VH

Gene Symbol

IGHM

Additional IgM Products

Product Documents for IgM Antibody (RM109) [Biotin]

Certificate of Analysis

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Product Specific Notices for IgM Antibody (RM109) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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