Rabbit anti-Feline IgM Heavy Chain Secondary Antibody [FITC]

Novus Biologicals | Catalog # NBP1-73836

Novus Biologicals

Key Product Details

Species Reactivity

Feline

Applications

Fluorophore-linked immunosorbent assay, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Polyclonal Rabbit IgG
View all IgM Heavy Chain Secondary Antibodies »

Product Specifications

Immunogen

Cat IgM mu heavy chain

Specificity

No reaction was observed against Cat IgG.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room

This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum, Cat IgM and Cat Serum

Scientific Data Images for Rabbit anti-Feline IgM Heavy Chain Secondary Antibody [FITC]

Flow Cytometry: Rabbit anti-Feline IgM Heavy Chain Secondary Antibody [FITC] [NBP1-73836] - Phenotyping of cat blood. Cat blood cells were washed and incubated with the antibody. It was used as a secondary antibody to check for bound IgM, in cat blood. It served as a B cell marker. This image was submitted via customer review.

Applications for Rabbit anti-Feline IgM Heavy Chain Secondary Antibody [FITC]

Application
Recommended Usage

Flow Cytometry

1:1:10 - 1:1000

Fluorophore-linked immunosorbent assay

1:10000 - 1:50000

Immunocytochemistry/ Immunofluorescence

1:1000 - 1:5000
Application Notes
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Reviewed Applications

Read 1 review rated 3 using NBP1-73836 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IgM Heavy Chain

Alternate Names

Constant region of heavy chain of IgM, Ig Mu Chain C Region BOT, Ig Mu Chain C Region GAL, Ig Mu Chain C Region OU, immunoglobulin heavy constant mu, Immunoglobulin mu chain

Gene Symbol

IGHM

Additional IgM Heavy Chain Products

Product Documents for Rabbit anti-Feline IgM Heavy Chain Secondary Antibody [FITC]

Certificate of Analysis

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Product Specific Notices for Rabbit anti-Feline IgM Heavy Chain Secondary Antibody [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Rabbit anti-Feline IgM Heavy Chain Secondary Antibody [FITC] (1)

3 out of 5
1 Customer Rating
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Customer Images

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  • Rabbit anti-Feline IgM Secondary Antibody [Fluorescein]
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Whole blood cells
    Species: Feline
    Verified Customer | Posted 02/01/2018
    Phenotyping of cat blood
    Cat blood cells were washed and incubated with the antibody. It was used as a secondary antibody to check for bound IgM, in cat blood. It served as a B cell marker.
    Rabbit anti-Feline IgM Heavy Chain Secondary Antibody [FITC] NBP1-73836

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FAQs

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