Equine IgM Isotype Control

Novus Biologicals | Catalog # NBP1-96970

Novus Biologicals
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Key Product Details

Species Reactivity

Equine

Applications

Western Blot, ELISA, SDS-Page

Label

Unconjugated

Antibody Source

Horse IgM

Format

Purified
View all IgM Isotype Controls »

Product Specifications

Specificity

No reaction was observed against anti-Horse IgG F(c). Some light chain cross reactivity will occur with anti-Horse IgG.

Host

Horse

Isotype

IgM

Description

This product was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Horse Serum and anti-Horse IgM (u chain specific)

Store vial at 4C prior to opening. This product is stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.

Scientific Data Images for Equine IgM Isotype Control

SDS-PAGE: Equine IgM Isotype Control [NBP1-96970]

SDS-PAGE: Equine IgM Isotype Control [NBP1-96970]

SDS-Page: Equine IgM Isotype Control [NBP1-96970] - SDS PAGE Results Equine IgM isotype control. Lane 1: Horse IgM Reduced 1X. Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Horse IgM Non-Reduced 1X. 4-20% Gel, Coomassie Stained.

Applications for Equine IgM Isotype Control

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

SDS-Page

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This product has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0

Format

Purified

Preservative

0.1% Sodium Azide

Concentration

Please contact technical services for concentration.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.

Background: IgM

IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to its large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to its high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences. IgM measurement yields information about the body's immediate resistance and response to infection as well as information related to specific diseases. Decreased levels are associated with immune deficiency states, hereditary deficiencies, and myeloma. Increased levels can be associated with Waldenstrom's macroglobulinemia, chronic infection and hepatocellular disease.

Long Name

Immunoglobulin M

Alternate Names

AGM1, DKFZp686I15196, DKFZp686I15212, FLJ00385, immunoglobulin heavy constant mu, MGC104996, MGC52291, MU, VH

Gene Symbol

IGHM

Additional IgM Products

Product Documents for Equine IgM Isotype Control

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Equine IgM Isotype Control

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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