Mouse IgM Isotype Control

Novus Biologicals | Catalog # NBP1-97007

Novus Biologicals

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, ELISA, SDS-Page

Label

Unconjugated

Antibody Source

Mouse IgM

Format

Purified
View all IgM Isotype Controls »

Product Specifications

Specificity

No reaction was observed against anti-Mouse IgG F(c). Some light chain cross-reactivity will occur with anti-Mouse IgG.

Host

Mouse

Isotype

IgM

Description

This product was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum and anti-Mouse IgM (u chain specific)

Store vial at 4C prior to opening. This product is stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.

Scientific Data Images for Mouse IgM Isotype Control

SDS-PAGE: Mouse IgM Isotype Control [NBP1-97007]

SDS-PAGE: Mouse IgM Isotype Control [NBP1-97007]

SDS-Page: Mouse IgM Isotype Control [NBP1-97007] - Lane 1: 5 uL Opal Prestained Marker. Lane 2: Reduced Mouse IgG Whole Molecule. Lane 3: Reduced Mouse F(c) Fragment. Lane 4: Reduced Mouse F(ab) Fragment. Lane 5: Mouse IgM Whole Molecule. Load: 1 ug per lane.
SDS-PAGE: Mouse IgM Isotype Control [NBP1-97007]

SDS-PAGE: Mouse IgM Isotype Control [NBP1-97007]

SDS-Page: Mouse IgM Isotype Control [NBP1-97007] - Lane 1: Mouse IgM, Non-Reduced. Lane 2: Mouse IgM, Reduced. Load: 1.0 ug per lane.

Applications for Mouse IgM Isotype Control

Application
Recommended Usage

ELISA

1:100 - 1:2000

SDS-Page

1:100 - 1:2000

Western Blot

1:100 - 1:2000
Application Notes
This product has been tested by SDS-Page and can be utilized as a control or standard reagent in SDS, Western Blotting, and ELISA experiments.

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0

Format

Purified

Preservative

0.1% Sodium Azide

Concentration

Please contact technical services for concentration.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.

Background: IgM

Mouse IgM, or mouse Immunoglobulin M, purified protein is a basic antibody that is produced by B cells. Mouse IgM is the primary antibody against A and B antigens on red blood cells. Mouse IgM is by far the physically largest antibody in the human circulatory system. Mouse IgM is the first antibody to appear in response to initial exposure to antigen.

Long Name

Immunoglobulin M

Alternate Names

AGM1, DKFZp686I15196, DKFZp686I15212, FLJ00385, immunoglobulin heavy constant mu, MGC104996, MGC52291, MU, VH

Gene Symbol

IGHM

Additional IgM Products

Product Documents for Mouse IgM Isotype Control

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Mouse IgM Isotype Control

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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