IGSF2/CD101 Antibody (9A8B9) - BSA Free

Novus Biologicals | Catalog # NBP3-27138

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 9A8B9

Format

BSA Free
View all IGSF2/CD101 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human IGSF2/CD101 (AA: extra 22-168) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

115 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IGSF2/CD101 Antibody (9A8B9) - BSA Free

Flow Cytometry: IGSF2/CD101 Antibody (9A8B9) [NBP3-27138] -

Flow Cytometry: IGSF2/CD101 Antibody (9A8B9) [NBP3-27138] - Analysis of HL-60 cells using IGSF2/CD101 mouse mAb (green) and negative control (red).

Western Blot: IGSF2/CD101 Antibody (9A8B9) [NBP3-27138] -

Western Blot: IGSF2/CD101 Antibody (9A8B9) [NBP3-27138] - Analysis using IGSF2/CD101 mAb against HEK293 (1) and IGSF2/CD101 (AA: extra 22-168)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot: IGSF2/CD101 Antibody (9A8B9) [NBP3-27138] -

Western Blot: IGSF2/CD101 Antibody (9A8B9) [NBP3-27138] - Analysis using IGSF2/CD101 mAb against human IGSF2/CD101 (AA: extra 22-168) recombinant protein. (Expected MW is 42.2 kDa)

ELISA: IGSF2/CD101 Antibody (9A8B9) [NBP3-27138] -

ELISA: IGSF2/CD101 Antibody (9A8B9) [NBP3-27138] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)

Applications for IGSF2/CD101 Antibody (9A8B9) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Western Blot

1:500 - 1:2000

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IGSF2/CD101

CD101 is a disulfide-linked homodimeric type 1 glycoprotein. The peptide is comprised of 7 extracellular V-type IgSF domains. CD101 is highly expressed on monocytes, granulocytes, mucosal T cells, and on activated peripheral blood T cells. Expression is weak on resting T and B and NK cells, absent from platelets and weak or absent from most hematopoietic cell lines. CD101 is thought to play a co-stimulatory role in TCR/CD3-mediated T cell activation. Monoclonal antibodies against CD101 inhibit allogeneic T cell responses. Studies suggest that CD101 plays a major role in the activation of T cells by skin dendritic cells.

Long Name

Immunoglobulin Superfamily, Member 2

Alternate Names

CD101, Leukocyte Surface Protein, V7

Gene Symbol

CD101

Additional IGSF2/CD101 Products

Product Documents for IGSF2/CD101 Antibody (9A8B9) - BSA Free

Certificate of Analysis

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Product Specific Notices for IGSF2/CD101 Antibody (9A8B9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Group 1 ILCs
Regulatory T Cells (Tregs)

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Protocols

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