IGSF2/CD101 Antibody (BB27) - BSA Free

Novus Biologicals | Catalog # NB100-65269

Clone BB27 was used by HLDA to establish CD designation.
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # BB27

Format

BSA Free
View all IGSF2/CD101 Primary Antibodies »

Product Specifications

Immunogen

Human thymic clone B12

Specificity

NB100-65269 recognizes human CD101. CD101 is a 140kD homodimeric cell surface glycoprotein expressed primarily by monocytes, granulocytes, dendritic cells and activated T lymphocytes. Studies suggest that CD101 plays a major role in the activation of T cells by skin dendritic cells. Clone BB27 has been reported to inhibit allogeneic T-cell responses (1). Removal of sodium azide is recommended prior to use in functional assays.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for IGSF2/CD101 Antibody (BB27) - BSA Free

Flow Cytometry: IGSF2/CD101 Antibody (BB27) - BSA Free [NB100-65269]

Flow Cytometry: IGSF2/CD101 Antibody (BB27) - BSA Free [NB100-65269]

Flow Cytometry: IGSF2/CD101 Antibody (BB27) [NB100-65269] - Figure A. FITC conjugated mouse anti human CD3 and RPE conjugated mouse IgG1 isotype control. Figure B. FITC conjugated mouse anti human CD3 and RPE conjugated mouse anti human CD101. All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of Human Seroblock.
Flow Cytometry: IGSF2/CD101 Antibody (BB27) - BSA Free [NB100-65269]

Flow Cytometry: IGSF2/CD101 Antibody (BB27) - BSA Free [NB100-65269]

Flow Cytometry: IGSF2/CD101 Antibody (BB27) [NB100-65269] - Figure A. FITC conjugated mouse anti human CD14 and RPE conjugated mouse IgG1 isotype control. Figure B. FITC conjugated mouse anti human CD14 and RPE conjugated mouse anti human CD101. All experiments performed on red cell lysed human blood gated on mononuclear cells in the presence of Human Seroblock.

Applications for IGSF2/CD101 Antibody (BB27) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IGSF2/CD101

CD101 is a disulfide-linked homodimeric type 1 glycoprotein. The peptide is comprised of 7 extracellular V-type IgSF domains. CD101 is highly expressed on monocytes, granulocytes, mucosal T cells, and on activated peripheral blood T cells. Expression is weak on resting T and B and NK cells, absent from platelets and weak or absent from most hematopoietic cell lines. CD101 is thought to play a co-stimulatory role in TCR/CD3-mediated T cell activation. Monoclonal antibodies against CD101 inhibit allogeneic T cell responses. Studies suggest that CD101 plays a major role in the activation of T cells by skin dendritic cells.

Long Name

Immunoglobulin Superfamily, Member 2

Alternate Names

CD101, Leukocyte Surface Protein, V7

Gene Symbol

CD101

UniProt

Q93033 (Human)

Additional IGSF2/CD101 Products

Product Documents for IGSF2/CD101 Antibody (BB27) - BSA Free

Certificate of Analysis

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Product Specific Notices for IGSF2/CD101 Antibody (BB27) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Group 1 ILCs
Regulatory T Cells (Tregs)

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Protocols

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