IKK alpha/beta Antibody (SR2996) - BSA Free

Novus Biologicals | Catalog # NBP3-27728

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR2996 expressed in HEK293

Format

BSA Free
View all IKK alpha/beta Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human IKK alpha/beta (Uniprot #: O15111/O14920)

Specificity

Detects endogenous levels of IKK alpha/beta

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IKK alpha/beta Antibody (SR2996) - BSA Free

Western Blot: IKK alpha/beta Antibody (SR2996) [NBP3-27728] -

Western Blot: IKK alpha/beta Antibody (SR2996) [NBP3-27728] - Western blot analysis of IKK alpha/beta expression in (1) A431 cell lysate; (2) 293T cell lysate; (3) Mouse kidney lysate.

Applications for IKK alpha/beta Antibody (SR2996) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunoprecipitation

1:50

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 150mM NaCl, 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IKK alpha/beta

The transcription factor NFkappaB is retained in the cytoplasm in an inactive form by the inhibitory protein IkappaB. Activation of NFkappaB requires that IkappaB be phosphorylated on specific serine residues, which results in targeted degradation of IkappaB. IkappaB kinase alpha (IKKalpha), previously designated CHUK, interacts with IkappaBalpha and specifically phosphorylates IkappaBalpha on Serine 32 and 36, the sites that trigger its degradation. IKKalpha appears to be critical for NFkappaB activation in response to proinflammatory cytokines. Phosphorylation of IkappaB by IKKalpha is stimulated by the NFkappaB inducing kinase (NIK), which itself is a central regulator for NFkappaB activation in response to TNF and IL-1. The functional IKK complex contains three subunits, IKKalpha, IKKbeta and IKKgamma (also designated NEMO), and each appear to make essential contributions to IkappaB phosphorylation.

Alternate Names

conserved helix-loop-helix ubiquitous kinaseIKBKA, EC 2.7.11, EC 2.7.11.10, IkappaB kinase, I-kappa-B kinase 1, I-kappa-B kinase alpha, IkBKA, IKK1I-kappa-B kinase-alpha, IKK-A, IKKAIkB kinase alpha subunit, IKK-alphainhibitor of nuclear factor kappa-B kinase subunit alpha, NFKBIKAIKK-a kinase, Nuclear factor NFkappaB inhibitor kinase alpha, Nuclear factor NF-kappa-B inhibitor kinase alpha, TCF16, TCF-16, Transcription factor 16

Gene Symbol

CHUK

Additional IKK alpha/beta Products

Product Documents for IKK alpha/beta Antibody (SR2996) - BSA Free

Certificate of Analysis

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Product Specific Notices for IKK alpha/beta Antibody (SR2996) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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