IL-12 (Interleukin-12) is a secreted heterodimeric cytokine that contains disulfide-linked p35 and p40 subunits. The p40 subunit is also a subunit of the heterodimeric IL-23. IL-12 is produced by macrophages, dendritic cells, monocytes, Langerhans cells, neutrophils, keratinocytes, microglia, and non-germinal center B cells. It acts through a receptor complex that contains the ligand binding IL-12 R alpha 1 and the signal transducing IL-12 R alpha 2. IL-12 can enhance cytotoxic activity and induce Interferon-gamma production in NK cells, T cells and dendritic epidermal T cells. In conjunction with IL-23 and IL-27, IL-12 promotes the development of a Th1 immune response.
IL-12/IL-23 p40 Antibody (171) [Janelia Fluor® 549]
Novus Biologicals | Catalog # NBP3-26107JF549
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Mouse
Applications
ELISA
Label
Janelia Fluor 549
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 171 expressed in HEK293
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Product Specifications
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse IL-12 p40/IL12B (Uniprot#: P43432; Met1-Ser335).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Applications for IL-12/IL-23 p40 Antibody (171) [Janelia Fluor® 549]
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: IL-12/IL-23 p40
Long Name
Interleukin 12/Interleukin 23 p40
Alternate Names
CLMF2, IL-12B, IL12 p40, IL12B, NKSF2
Gene Symbol
IL12B
Additional IL-12/IL-23 p40 Products
Product Documents for IL-12/IL-23 p40 Antibody (171) [Janelia Fluor® 549]
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for IL-12/IL-23 p40 Antibody (171) [Janelia Fluor® 549]
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for IL-12/IL-23 p40 Antibody (171) [Janelia Fluor® 549]
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Q: What is the difference between the Mouse IL-12 p40 Allele-specific (M1240) and Non Allele-specific(MP400) Quantikine ELISA kits?
A: Polymorphisms exist in the mouse IL-12/IL-23 p40 sequence. The monoclonal capture antibody used in M1240 is allele-specific. It recognizes the IL-12/IL-23 p40 variant with methionine at residue 169 (Accession # P43432) in mouse strains A.SW, BALB/c, C3H, DBA/1, C57BL/6, NOR, and 129/J. The MP400 kit recognizes variant and non-variant mouse IL-12/IL-23 p40.
