IL-12 (Interleukin-12) is a secreted heterodimeric cytokine that contains disulfide-linked p35 and p40 subunits. The p40 subunit is also a subunit of the heterodimeric IL-23. IL-12 is produced by macrophages, dendritic cells, monocytes, Langerhans cells, neutrophils, keratinocytes, microglia, and non-germinal center B cells. It acts through a receptor complex that contains the ligand binding IL-12 R alpha 1 and the signal transducing IL-12 R alpha 2. IL-12 can enhance cytotoxic activity and induce Interferon-gamma production in NK cells, T cells and dendritic epidermal T cells. In conjunction with IL-23 and IL-27, IL-12 promotes the development of a Th1 immune response.
Rat IL-12/IL-23 p40 ELISA Kit (Colorimetric)
Novus Biologicals | Catalog # NBP3-06741
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Key Product Details
Sample Type & Volume Required Per Well
Serum, plasma, cell supernatant, and other biological fluids (100 ul)
Sensitivity
7.5 pg/mL
Assay Range
12.5 - 800 pg/mL
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Product Specifications
Assay Type
Sandwich-ELISA
Kit Type
ELISA Kit (Colorimetric)
Species
Rat
Specificity
This kit recognizes Rat IL-Y (IL-12p40/IL-27p28) in samples. No significant cross-reactivity or interference between Rat IL-Y (IL-12p40/IL-27p28) and analogues was observed.
Precision
Intra-Assay Precision (Precision within an assay) 4.72
Inter-Assay Precision (Precision between assays) 4.8
Recovery for Rat IL-12/IL-23 p40 ELISA Kit (Colorimetric)
Recovery
85 - 102 %
Linearity
Scientific Data Images for Rat IL-12/IL-23 p40 ELISA Kit (Colorimetric)
ELISA: Rat IL-12/IL-23 p40 ELISA Kit (Colorimetric) [NBP3-06741] -
ELISA: Rat IL-12/IL-23 p40 ELISA Kit (Colorimetric) [NBP3-06741] - Standard Curve ReferenceKit Contents for Rat IL-12/IL-23 p40 ELISA Kit (Colorimetric)
- Biotinylated Detection Ab Diluent
- Concentrated Biotinylated Detection Ab (100x)
- Concentrated HRP Conjugate (100x)
- Concentrated Wash Buffer (25x)
- HRP Conjugate Diluent
- Micro ELISA Plate (Dismountable)
- Plate Sealer
- Product Manual
- Reference Standard
- Sample Diluent
- Stop Solution
- Substrate Reagent
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: IL-12/IL-23 p40
Long Name
Interleukin 12/Interleukin 23 p40
Alternate Names
CLMF2, IL-12B, IL12 p40, IL12B, NKSF2
Gene Symbol
IL12B
Additional IL-12/IL-23 p40 Products
Product Documents for Rat IL-12/IL-23 p40 ELISA Kit (Colorimetric)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Rat IL-12/IL-23 p40 ELISA Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
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- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Rat IL-12/IL-23 p40 ELISA Kit (Colorimetric)
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Q: What is the difference between the Mouse IL-12 p40 Allele-specific (M1240) and Non Allele-specific(MP400) Quantikine ELISA kits?
A: Polymorphisms exist in the mouse IL-12/IL-23 p40 sequence. The monoclonal capture antibody used in M1240 is allele-specific. It recognizes the IL-12/IL-23 p40 variant with methionine at residue 169 (Accession # P43432) in mouse strains A.SW, BALB/c, C3H, DBA/1, C57BL/6, NOR, and 129/J. The MP400 kit recognizes variant and non-variant mouse IL-12/IL-23 p40.
