IL-17RD/SEF Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32307

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all IL-17RD/SEF Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human IL-17RD/SEF. The exact sequence is proprietary.

Localization

Golgi apparatus membrane, Single-pass type I membrane protein, Cell membrane, Single-pass type I membrane protein, Isoform 4: Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

82 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IL-17RD/SEF Antibody - BSA Free

Western Blot: IL-17RD/SEF Antibody [NBP1-32307]

Western Blot: IL-17RD/SEF Antibody [NBP1-32307]

Western Blot: IL-17 RD/SEF Antibody [NBP1-32307] - Sample(30 ug of whole cell lysate)A:293T7. 5% SDS PAGE, antibody diluted at 1:500.
Western Blot: IL-17RD/SEF Antibody [NBP1-32307]

Western Blot: IL-17RD/SEF Antibody [NBP1-32307]

Western Blot: IL-17 RD/SEF Antibody [NBP1-32307] - (30 ug of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE diluted at 1:2000
Western Blot: IL-17RD/SEF Antibody [NBP1-32307]

Western Blot: IL-17RD/SEF Antibody [NBP1-32307]

Western Blot: IL-17 RD/SEF Antibody [NBP1-32307] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.

Applications for IL-17RD/SEF Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: IL-17RD/SEF

Fibroblast growth factors (FGFs; see MIM 603726) are secreted proteins involved in cellular proliferation, migration, differentiation, and survival. FGF activity is negatively regulated by members of the 'sprouty' family (e.g., SPRY1, MIM 602465). The SEF protein is a modulator of FGF signaling.[supplied by OMIM]

Long Name

Interleukin 17 Receptor D, Transcript Variant 3

Alternate Names

IL-17 RD, IL17RD, IL17RLM, SEF

Gene Symbol

IL17RD

Additional IL-17RD/SEF Products

Product Documents for IL-17RD/SEF Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for IL-17RD/SEF Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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FAQs for IL-17RD/SEF Antibody - BSA Free

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  • Q: We are interested in your antibody IL17RD Antibody (NBP1-32307). However, we have a question regarding it. There are two WB images on your website, where you show the detection of IL17RD. There are two WB images on your website, where you show the detection of IL17RD. On the first image you point to an approximately 80kDa band in HEK293T cells. Is this a transfected protein or the endogenous one? If this is the endogeous IL17RD, why is it 80kDa, and not approximately 120kDa, as it is expected from a glycosylated transmembrane IL17RD? Or is this the cytosolic isoform 4 of IL17RD? Could you clarify this for us?

    A: In regards to your inquiry the HEK293T cells are transfected with the protein as an overexpression lysate. This product however is also tested on endogenous samples of 3T3 and Mouse brain. In those samples a band of similar size was also observed. Despite the glycosylation of this protein we detected it at approx. 79 kDa in our samples. The antibody should not be specific for just isoform 4. Since it is picking up at the lower molecular weight it may be detecting the cytosolic form with more prevalence than the membrane bound protein since whole cell preps were used and not just membrane preps.

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