Human/mouse IL-28A (IFN-lambda 2), IL-28B (IFN-lambda 3), and human IL-29 (IFN-lambda 1) are class II cytokine receptor ligands that are distantly related to members of the IL-10 family (11 - 13% amino acid (aa) sequence identity) and type I IFN family (15 - 19% aa sequence identity). These cytokines exert bioactivities that overlap those of type I IFNs, including antiviral activity and up-regulation of MHC class I antigen expression. The proteins signal through the same heterodimeric receptor complex that is composed of the IL-10 receptor beta (IL-10 R beta) and a novel IL-28 receptor alpha (IL-28 R alpha, also known as IFN-lambda R1). Mouse IL-28 shares 61%, 62%, and 52% aa identity with human IL-28A, IL-28B, and IL-29, respectively.
IL-28B/IFN-lambda 3 Antibody (244710) [FITC]
Novus Biologicals | Catalog # FAB1789F
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot, Neutralization
Label
FITC (Excitation = 495 nm, Emission = 519 nm)
Antibody Source
Monoclonal Rat IgG2B Clone # 244710
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Product Specifications
Immunogen
E. coli-derived recombinant mouse IL‑28B/IFN‑ lambda 3
Asp20-Val193
Accession # Q8CGK6
Asp20-Val193
Accession # Q8CGK6
Specificity
Detects mouse IL‑28B/IFN‑ lambda 3 in direct ELISAs and Western blots. In Western blots, approximately 25% cross-reactivity with recombinant mouse (rm) IL-28A/IFN-gamma 2 and no cross-reactivity with recombinant human (rh) IL-28A or rhIL-29 is observed. This antibody neutralizes 100% of the bioactivity of rmIL-28A/IFN-gamma 2.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Applications for IL-28B/IFN-lambda 3 Antibody (244710) [FITC]
Application
Recommended Usage
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Neutralization
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: IL-28B/IFN-lambda 3
Long Name
Interleukin 28B
Alternate Names
IFN-lambda 3
Gene Symbol
IFNL3
Additional IL-28B/IFN-lambda 3 Products
Product Documents for IL-28B/IFN-lambda 3 Antibody (244710) [FITC]
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for IL-28B/IFN-lambda 3 Antibody (244710) [FITC]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
