Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all IL-29/IFN-lambda 1 Primary Antibodies »

Product Specifications

Immunogen

This IgG fraction IL-29/IFN-lambda 1 Antibody was prepared from rabbit antiserum after repeated immunizations with mature length recombinant human IL-29/IFN-lambda 1/IFN-lambda 1 protein produced in E.coli. (Uniprot: Q8IU54)

Reactivity Notes

A BLAST analysis was used to suggest cross-reactivity with IL-29/IFN-lambda 1 from human sources based on 100% homology with the immunizing sequence. Based on high to 100% homology, there is a chance of cross-reactivity to IL-29/IFN-lambda 1 from a wide variety of animals. Cross-reactivity with IL-29/IFN-lambda 1 from other sources has not been determined.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above

Store this antibody at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for IL-29/IFN-lambda 1 Antibody - Azide and BSA Free

Western Blot: IL-29/IFN-lambda 1 Antibody [NBP1-77893]

Western Blot: IL-29/IFN-lambda 1 Antibody [NBP1-77893]

Western Blot: IL-29/IFN-lambda 1 Antibody [NBP1-77893] - Shows detection of recombinant human IL-29 raised in E.coli. Recombinant protein (0.1 ug, 19.9 kDa) was loaded onto and resolved by SDS-PAGE, then transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Anti-Human IL-29. After washing, membrane was probed with secondary antibody DyLight (TM) 649 conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody diluted 1:20,000 in blocking buffer for 30 min. at RT.

Applications for IL-29/IFN-lambda 1 Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

1:10000

Western Blot

1:1000
Application Notes
This purified antibody has been tested in western blotting and suitable for ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 19.9 kDa in size corresponding to the mature human IL-29 protein by western blotting in appropriate cell lysate or extract.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-29/IFN-lambda 1

IL-29 (Interleukin-29) is a secreted cytokine with immunomodulatory activity. It may play a role in antiviral immunity. It up-regulates MHC class I antigen expression. IL-29 is a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IL28RA. The ligand/receptor complex seems to signal through the Jak-STAT pathway. Its induction is by viral infections or double stranded RNA.

Long Name

Interleukin 29

Alternate Names

IFN-lambda 1, IL29

Gene Symbol

IFNL1

UniProt

Q8IU54, NP_742152.1 (Human)

Additional IL-29/IFN-lambda 1 Products

Product Documents for IL-29/IFN-lambda 1 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for IL-29/IFN-lambda 1 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Interferons

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FAQs

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Associated Pathways

Toll-Like Receptor Signaling Pathways Toll-Like Receptor Signaling Pathway Thumbnail