IL-36Ra/IL-1F5 Antibody Pair [Biotin]

Sandwich ELISA

0.78 - 50 ng/ml

Cell Culture Supernatant, Serum (100 ul)

0.5ng/ml

Human

Sandwich ELISA for the quantitative determination of Human IL-36Ra/IL-1F5

100 ul

Biotin

1. Dilute the desired amount of Coating Antibody (COAT) (1 mg/ml) (mouse monoclonal antibody) to 5 ug/ml in PBS without carrier protein and use it fresh.
2. Dilute the desired amount of Detection Antibody (DET) (0.5 mg/ml) (biotinylated mouse monoclonal antibody) to 1 ug/ml in ELISA Buffer and use it fresh.
3. Reconstitute the Streptavidin-HRP (STREP) with 100 ul ELISA Buffer. After reconstitution, prepare aliquots and store the reconstituted STREP at -20C. Avoid freeze/thaw cycles. Dilute the reconstituted Streptavidin-HRP to the working concentration by adding 10 ul in 10 ml of ELISA Buffer (1:1000).
4. Reconstitute the Standard Protein (STD) (recombinant human IL-36Ra/IL-1F5) with 100 ul ELISA Buffer to obtain a concentration of 10 ug/ml. After reconstitution, prepare aliquots and store the reconstituted standard at -20C. Avoid freeze/ thaw cycles. A standard curve using 2-fold serial dilutions in ELISA Buffer is recommended. Suggested standard points are 50 ng/ml, 25 ng/ml, 12.50 ng/ml, 6.25 ng/ml, 3.12 ng/ml, 1.56 ng/ml, 0.78 ng/ml and 0 ng/ml.

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.