IMP2/IGF2BP2 Antibody

Novus Biologicals | Catalog # NB100-93408

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Mouse (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all IMP2 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KQQEQKYPQGVASQR corresponding to C-Terminus according to NP_006539.3, NP_001007226.1.

Specificity

This antibody is expected to recognise both reported isoforms (NP_006539.3 and NP_001007226.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IMP2/IGF2BP2 Antibody

Western Blot: IMP2/IGF2BP2 Antibody [NB100-93408]

Western Blot: IMP2/IGF2BP2 Antibody [NB100-93408]

Western Blot: IGF2BP2 Antibody [NB100-93408] - HEK293 overexpressing IGF2BP2 and probed with (mock transfection in first lane).
Western Blot: IMP2/IGF2BP2 Antibody [NB100-93408]

Western Blot: IMP2/IGF2BP2 Antibody [NB100-93408]

Western Blot: IGF2BP2 Antibody [NB100-93408] - (1ug/ml) staining of HepG2 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for IMP2/IGF2BP2 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 60-70 kDa band observed in HepG2 lysates (calculated MW of 66.1 kDa band according to NP_006539.3). In transfected HEK293 transiently expressing IGF2BP2 a band of approx. 65 kDa is observed. This band is not observed in the non-transfected HEK293.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IMP2

This gene encodes a member of the IGF-II mRNA-binding protein (IMP) family. The protein encoded by this gene contains several four KH domains and two RRM domains. It functions by binding to the 5' UTR of the insulin-like growth factor 2 (IGF2) mRNA and regulating IGF2 translation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Long Name

Insulin-like Growth Factor 2 mRNA Binding Protein 2

Alternate Names

IF2B2, IGF2BP2, VICKZ2

Entrez Gene IDs

10644 (Human); 319765 (Mouse)

Gene Symbol

IGF2BP2

UniProt

Q9Y6M1

Additional IMP2 Products

Product Documents for IMP2/IGF2BP2 Antibody

Certificate of Analysis

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Product Specific Notices for IMP2/IGF2BP2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for IMP2/IGF2BP2 Antibody

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