Importin beta/KPNB1 Antibody

Name: Importin beta/KPNB1 Antibody
Brand: Novus Biologicals
SKU: NB100-40784
Price: 399 USD
Availability: InStock

Novus Biologicals | Catalog # NB100-40784

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

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Product Specifications

Immunogen

Peptide with sequence C-RRSKTNKAKTLAT corresponding to C-Terminus according to NP_002256.2.

Reactivity Notes

Expected from sequence similarity: Mouse, Canine, Bovine

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Importin beta/KPNB1 Antibody

Western Blot: Importin beta/KPNB1 Antibody [NB100-40784]

Western Blot: Importin beta/KPNB1 Antibody [NB100-40784] - Staining of A431 (A), HEK293 (B), Jurkat (C) cell lysates with antibody at 0.03 ug/mL, Daudi (D), HeLa (E) cell lysates with antibody at 0.01 ug/mL, and Kelly (F) and KNRK (G) cell lysate with antibody at 0.1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.

Immunocytochemistry/ Immunofluorescence: Importin beta/KPNB1 Antibody [NB100-40784]

Immunocytochemistry/Immunofluorescence: Importin beta/KPNB1 Antibody [NB100-40784] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear membrane, nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Immunohistochemistry-Paraffin: Importin beta/KPNB1 Antibody [NB100-40784]

Immunohistochemistry-Paraffin: Importin beta/KPNB1 Antibody [NB100-40784] - Staining of paraffin embedded Human Spleen with antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Flow Cytometry: Importin beta/KPNB1 Antibody [NB100-40784]

Flow Cytometry: Importin beta/KPNB1 Antibody [NB100-40784] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

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Applications for Importin beta/KPNB1 Antibody

Application

Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunohistochemistry

5 ug/mL

Immunohistochemistry-Paraffin

5 ug/mL

Peptide ELISA

Detection limit 1:32000

Western Blot

0.01 - 0.1 ug/mL

Flow Cytometry Panel Builder

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Importin beta/KPNB1

Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family.

Long Name

Karyopherin beta 1

Alternate Names

IMB1, Impnb, Importin 1, IPOB, KPNB1, NTF97, PTAC97

Entrez Gene IDs

3837 (Human); 16211 (Mouse); 24917 (Rat)

Gene Symbol

KPNB1

UniProt

NP_002256.2 (Human)

Additional Importin beta/KPNB1 Products

Product Documents for Importin beta/KPNB1 Antibody

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Certificate of Analysis

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Product Specific Notices for Importin beta/KPNB1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Importin beta/KPNB1 Antibody

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