INCENP Antibody (3D2) - BSA Free

Novus Biologicals | Catalog # NBP2-37471

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3D2

Format

BSA Free
View all INCENP Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human INCENP (AA: 369-583) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

105.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for INCENP Antibody (3D2) - BSA Free

Western Blot: INCENP Antibody (3D2)BSA Free [NBP2-37471]

Western Blot: INCENP Antibody (3D2)BSA Free [NBP2-37471]

Western Blot: INCENP Antibody (3D2) [NBP2-37471] - Analysis using INCENP mAb against HEK293 (1) and INCENP (AA: 369-583)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: INCENP Antibody (3D2) - BSA Free [NBP2-37471]

Immunocytochemistry/ Immunofluorescence: INCENP Antibody (3D2) - BSA Free [NBP2-37471]

Immunocytochemistry/Immunofluorescence: INCENP Antibody (3D2) [NBP2-37471] - Immunofluorescence analysis of HepG2 cells using INCENP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry-Paraffin: INCENP Antibody (3D2) - BSA Free [NBP2-37471]

Immunohistochemistry-Paraffin: INCENP Antibody (3D2) - BSA Free [NBP2-37471]

Immunohistochemistry-Paraffin: INCENP Antibody (3D2) [NBP2-37471] - Analysis of rectum cancer tissues using INCENP mouse mAb with DAB staining.
Flow Cytometry: INCENP Antibody (3D2) - BSA Free [NBP2-37471]

Flow Cytometry: INCENP Antibody (3D2) - BSA Free [NBP2-37471]

Flow Cytometry: INCENP Antibody (3D2) [NBP2-37471] - Analysis of Jurkat cells using INCENP mouse mAb (green) and negative control (red).
Western Blot: INCENP Antibody (3D2)BSA Free [NBP2-37471]

Western Blot: INCENP Antibody (3D2)BSA Free [NBP2-37471]

Western Blot: INCENP Antibody (3D2) [NBP2-37471] - Analysis using INCENP mAb against human INCENP recombinant protein. (Expected MW is 50.2 kDa)
Immunohistochemistry-Paraffin: INCENP Antibody (3D2) - BSA Free [NBP2-37471]

Immunohistochemistry-Paraffin: INCENP Antibody (3D2) - BSA Free [NBP2-37471]

Immunohistochemistry-Paraffin: INCENP Antibody (3D2) [NBP2-37471] - Analysis of cervical cancer tissues using INCENP mouse mAb with DAB staining.
ELISA: INCENP Antibody (3D2) - BSA Free [NBP2-37471]

ELISA: INCENP Antibody (3D2) - BSA Free [NBP2-37471]

ELISA: INCENP Antibody (3D2) [NBP2-37471] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for INCENP Antibody (3D2) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: INCENP

In mammalian cells, 2 broad groups of centromere-interacting proteins have been described: constitutively binding centromere proteins and 'passenger,' or transiently interacting, proteins (reviewed by Choo, 1997). The constitutive proteins include CENPA (centromere protein A; MIM 117139), CENPB (MIM 117140), CENPC1 (MIM 117141), and CENPD (MIM 117142). The term 'passenger proteins' encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle (Earnshaw and Mackay, 1994). These include CENPE (MIM 117143); MCAK (MIM 604538); KID (MIM 603213); cytoplasmic dynein (e.g., MIM 600112); CliPs (e.g., MIM 179838); and CENPF/mitosin (MIM 600236). The inner centromere proteins (INCENPs) (Earnshaw and Cooke, 1991), the initial members of the passenger protein group, display a broad localization along chromosomes in the early stages of mitosis but gradually become concentrated at centromeres as the cell cycle progresses into mid-metaphase. During telophase, the proteins are located within the midbody in the intercellular bridge, where they are discarded after cytokinesis (Cutts et al., 1999).[supplied by OMIM]

Alternate Names

binds and activates aurora-B and -C in vivo and in vitro, chromosomal passenger protein, FLJ31633, inner centromere protein, inner centromere protein antigens (135kD, 155kD), inner centromere protein antigens 135/155kDa, inner centromere protein INCENP, MGC111393

Gene Symbol

INCENP

Additional INCENP Products

Product Documents for INCENP Antibody (3D2) - BSA Free

Certificate of Analysis

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Product Specific Notices for INCENP Antibody (3D2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for INCENP Antibody (3D2) - BSA Free

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Protocols

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