Indian Hedgehog/Ihh Antibody (9L16) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-22020

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2 Clone # 9L16

Format

Azide and BSA Free
View all Indian Hedgehog/Ihh Primary Antibodies »

Product Specifications

Immunogen

Human recombinant Indian Hedgehog/Ihh (N-terminus)

Clonality

Monoclonal

Host

Rat

Isotype

IgG2

Scientific Data Images for Indian Hedgehog/Ihh Antibody (9L16) - Azide and BSA Free

Western Blot: Indian Hedgehog/Ihh Antibody (9L16)Azide and BSA Free [NBP2-22020]

Western Blot: Indian Hedgehog/Ihh Antibody (9L16)Azide and BSA Free [NBP2-22020]

Western Blot: Indian Hedgehog/Ihh Antibody (9L16) [NBP2-22020] - Lysates of HEK293 expressing human IHH N-terminus.

Applications for Indian Hedgehog/Ihh Antibody (9L16) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:2000

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Reconstitution

Reconstitute with sterilized PBS to a final concentration of 0.2 mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Indian Hedgehog/Ihh

Hedgehog, WNT, FGF and BMP signaling pathways network together during embryogenesis, tissue regeneration, and carcinogenesis. Aberrant activation of Hedgehog signaling pathway leads to pathological consequences in a variety of human tumors, such as gastric cancer and pancreatic cancer (1). The hedgehog family of morphogens are regulators of cell proliferation, differentiation and cell-cell communication. These morphogens have been shown to have important roles in organogenesis, spermatogenesis, stem cell maintenance and oncogenesis. Indian hedgehog (IHH) has been shown to be expressed in the uterine epithelium under the control of the steroid hormone, progesterone (2). It has been shown that IHH, synthesized by prehypertrophic and hypertrophic chondrocytes, regulates the site of hypertrophic differentiation by signaling to the periarticular growth plate and also determines the site of bone collar formation in the adjacent perichondrium. By providing crucial local signals from prehypertrophic and hypertrophic chondrocytes to both chondrocytes and preosteoblasts, IHH couples chondrogenesis to osteogenesis in endochondral bone development (3).

Alternate Names

BDA1, HHG2, Ihh

Gene Symbol

IHH

UniProt

NP_002172 (Human)

Additional Indian Hedgehog/Ihh Products

Product Documents for Indian Hedgehog/Ihh Antibody (9L16) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Indian Hedgehog/Ihh Antibody (9L16) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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