iNOS Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-97471

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat, Bovine

Cited:

Mouse, Bovine

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all iNOS Primary Antibodies »

Product Specifications

Immunogen

iNOS Antibody was made to a synthetic peptide corresponding to amino aicds 1131-1144 of mouse iNOS.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

131 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for iNOS Antibody - BSA Free

Western Blot: iNOS Antibody [NBP1-97471]

Western Blot: iNOS Antibody [NBP1-97471]

Western Blot: iNOS Antibody [NBP1-97471] - Rat brain extract, probed with iNOS, pAb.

Applications for iNOS Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

1:10-1:500

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS and 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: iNOS

Nitric oxide (NO) is a colorless, free radical gas that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family including the two constitutive isoforms: brain, bNOS, or neuronal NOS, nNOS (type I) and endothelial cell NOS, eNOS (type III); along with the inducible isoform, iNOS (type II). NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO, requiring the cofactors calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN), heme, and tetrahydrobiopterin (1).

The 131 kDa enzyme, iNOS, is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. While constitutively expressed in kidneys, in other tissues iNOS is induced by bacterial lipopolysaccharides (LPS), growth factors, and cytokines such as IFN-gamma, TNF, IL-1 and IL-2. iNOS is not regulated by the level of intracellular Ca2+ and is constantly active as a dimer when expressed. iNOS activity is elevated in a variety of diseases including atherosclerosis, heart failure, sepsis, solid tumors, and type 2 diabetes. Acting as a critical mediator of inflammation and apoptosis, iNOS inhibitors have been shown to alleviate obesity and stress inducted insulin resistance in mouse models (2,3).

References

1. Forstermann U, and Sessa WC. (2012) Nitric oxide synthases: regulation and function. Eur Heart J. 33(7): 829-837. PMID: 21890489

2. Aktan F. (2004) iNOS-mediated nitric oxide production and its regulation. Life Sci. 75(6):639-53. PMID: 15172174

3. Cinelli MA, Do HT, Miley GP, Silverman RB. (2020) Inducible nitric oxide synthase: Regulation, structure, and inhibition. Med Res Rev. 40(1):158-189. PMID: 31192483

Long Name

Inducible Nitic Oxide Synthase

Alternate Names

NOS2, NOS2A

Entrez Gene IDs

18126 (Mouse); 24599 (Rat); 282876 (Bovine)

Gene Symbol

NOS2

Additional iNOS Products

Product Documents for iNOS Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for iNOS Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for iNOS Antibody - BSA Free

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FAQs for iNOS Antibody - BSA Free

Showing  1 - 4 of 4 FAQs Showing All

  • Q: I used NBP1-97471 antibody to run WB on cow mammary epithelial cells treated by LPS. I used 60ug/lane, 10% gel, and I found there was no specific band. Would you please give some advice?

    A: I think you can try running slightly less protein/lane. We usually use 20-30ug, so you can try 30-40ug instead of 60ug. The 10% gel should be fine, but if you want to separate the higher weights more, you can try using a lower percentage gel.

  • Q: We need to evaluate the level of iNOS in Rat sciatic nerve by Western Blot. I see that you have few antibodies suitable for that, could you please recommend the best what you have for our purposes?

    A:

    We have 4 iNOS antibodies that are suitable for your experiment. The best choice for you will depend on your specific preferences, in this case the main difference will be the immunogen location. If the immunogen is not important to you, then I would recommend choosing NB300-605 as it gives you the best value and we are able to provide the exact immunogen.

  • Q: What is the concentration of NBP1-97471?

    A: The concentration for this product (lot 01061105) is 1.0mg/ml.

  • Q: Would you please confirm the concentration of the current lot of this Ab: iNOS Antibody (NBP1-97471) - 0.05mg

    A: The antibody is supplied at 1mg/ml.

  • Q: I used NBP1-97471 antibody to run WB on cow mammary epithelial cells treated by LPS. I used 60ug/lane, 10% gel, and I found there was no specific band. Would you please give some advice?

    A: I think you can try running slightly less protein/lane. We usually use 20-30ug, so you can try 30-40ug instead of 60ug. The 10% gel should be fine, but if you want to separate the higher weights more, you can try using a lower percentage gel.

  • Q: We need to evaluate the level of iNOS in Rat sciatic nerve by Western Blot. I see that you have few antibodies suitable for that, could you please recommend the best what you have for our purposes?

    A:

    We have 4 iNOS antibodies that are suitable for your experiment. The best choice for you will depend on your specific preferences, in this case the main difference will be the immunogen location. If the immunogen is not important to you, then I would recommend choosing NB300-605 as it gives you the best value and we are able to provide the exact immunogen.

  • Q: What is the concentration of NBP1-97471?

    A: The concentration for this product (lot 01061105) is 1.0mg/ml.

  • Q: Would you please confirm the concentration of the current lot of this Ab: iNOS Antibody (NBP1-97471) - 0.05mg

    A: The antibody is supplied at 1mg/ml.

  • Q: I used NBP1-97471 antibody to run WB on cow mammary epithelial cells treated by LPS. I used 60ug/lane, 10% gel, and I found there was no specific band. Would you please give some advice?

    A: I think you can try running slightly less protein/lane. We usually use 20-30ug, so you can try 30-40ug instead of 60ug. The 10% gel should be fine, but if you want to separate the higher weights more, you can try using a lower percentage gel.

  • Q: We need to evaluate the level of iNOS in Rat sciatic nerve by Western Blot. I see that you have few antibodies suitable for that, could you please recommend the best what you have for our purposes?

    A:

    We have 4 iNOS antibodies that are suitable for your experiment. The best choice for you will depend on your specific preferences, in this case the main difference will be the immunogen location. If the immunogen is not important to you, then I would recommend choosing NB300-605 as it gives you the best value and we are able to provide the exact immunogen.

  • Q: What is the concentration of NBP1-97471?

    A: The concentration for this product (lot 01061105) is 1.0mg/ml.

  • Q: Would you please confirm the concentration of the current lot of this Ab: iNOS Antibody (NBP1-97471) - 0.05mg

    A: The antibody is supplied at 1mg/ml.

  • Q: I used NBP1-97471 antibody to run WB on cow mammary epithelial cells treated by LPS. I used 60ug/lane, 10% gel, and I found there was no specific band. Would you please give some advice?

    A: I think you can try running slightly less protein/lane. We usually use 20-30ug, so you can try 30-40ug instead of 60ug. The 10% gel should be fine, but if you want to separate the higher weights more, you can try using a lower percentage gel.

  • Q: We need to evaluate the level of iNOS in Rat sciatic nerve by Western Blot. I see that you have few antibodies suitable for that, could you please recommend the best what you have for our purposes?

    A:

    We have 4 iNOS antibodies that are suitable for your experiment. The best choice for you will depend on your specific preferences, in this case the main difference will be the immunogen location. If the immunogen is not important to you, then I would recommend choosing NB300-605 as it gives you the best value and we are able to provide the exact immunogen.

  • Q: What is the concentration of NBP1-97471?

    A: The concentration for this product (lot 01061105) is 1.0mg/ml.

  • Q: Would you please confirm the concentration of the current lot of this Ab: iNOS Antibody (NBP1-97471) - 0.05mg

    A: The antibody is supplied at 1mg/ml.

Showing  1 - 4 of 4 FAQs Showing All
View all FAQs for Antibodies