INSIG-2 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-03000

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all INSIG-2 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 50-150 of human INSIG-2 (NP_057217.2). QIQRNVTLFPPDVIASIFSSAWWVPPCCGTASAVIGLLYPCIDRHLGEPHKFKREWSSVMRCVAVFVGINHASAKVDFDNNIQLSLTLAALSIGLWWTFDR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for INSIG-2 Antibody - Azide and BSA Free

Western Blot: INSIG-2 AntibodyAzide and BSA Free [NBP3-03000]

Western Blot: INSIG-2 AntibodyAzide and BSA Free [NBP3-03000]

Western Blot: INSIG-2 Antibody [NBP3-03000] - Analysis of extracts of Mouse kidney, using INSIG-2 antibody (NBP3-03000) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry-Paraffin: INSIG-2 Antibody - Azide and BSA Free [NBP3-03000]

Immunohistochemistry-Paraffin: INSIG-2 Antibody - Azide and BSA Free [NBP3-03000]

Immunohistochemistry-Paraffin: INSIG-2 Antibody [NBP3-03000] - Rat ovary using INSIG-2 Rabbit pAb (NBP3-03000) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Western Blot: INSIG-2 AntibodyAzide and BSA Free [NBP3-03000]

Western Blot: INSIG-2 AntibodyAzide and BSA Free [NBP3-03000]

Western Blot: INSIG-2 Antibody [NBP3-03000] - Analysis of extracts of various cell lines, using INSIG-2 antibody (NBP3-03000) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry-Paraffin: INSIG-2 Antibody - Azide and BSA Free [NBP3-03000]

Immunohistochemistry-Paraffin: INSIG-2 Antibody - Azide and BSA Free [NBP3-03000]

Immunohistochemistry-Paraffin: INSIG-2 Antibody [NBP3-03000] - Mouse brain using INSIG-2 Rabbit pAb (NBP3-03000) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Applications for INSIG-2 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: INSIG-2

The protein encoded by the INSIG2 gene is highly similar to the protein product encoded by gene INSIG1. Both INSIG1 proteinand this protein are endoplasmic reticulum proteins that block the processing of sterol regulatory element bindingproteins (SREBPs) by binding to SREBP cleavage-activating protein (SCAP), and thus prevent SCAP from escorting SREBPsto the Golgi. (provided by RefSeq)

Alternate Names

INSIG-2, INSIG2 membrane protein, insulin induced gene 2, insulin induced protein 2, insulin-induced gene 2 protein, MGC26273

Gene Symbol

INSIG2

Additional INSIG-2 Products

Product Documents for INSIG-2 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for INSIG-2 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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