Integrin alpha L/CD11a Antibody

Name: Integrin alpha L/CD11a Antibody
Brand: Novus Biologicals
SKU: NBP1-51967
Price: 399 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-51967

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

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Product Specifications

Immunogen

Peptide with sequence C-YQVRIQPSIHDHN corresponding to internal region according to NP_002200.2, NP_001107852.1.

Epitope

C-YQVRIQPSIHDHN

Specificity

This antibody is expected to recognize both reported isoforms (NP_002200.2; NP_001107852.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Integrin alpha L/CD11a Antibody

Western Blot: Integrin alpha L/CD11a Antibody [NBP1-51967]

Western Blot: Integrin alpha L/CD11a Antibody [NBP1-51967] - (0.5ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Integrin alpha L/CD11a Antibody [NBP1-51967]

Immunohistochemistry-Paraffin: Integrin alpha L/CD11a Antibody [NBP1-51967] - (2ug/ml) staining of paraffin embedded Human Lymph Node. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

Immunohistochemistry-Paraffin: Integrin alpha L/CD11a Antibody [NBP1-51967]

Immunohistochemistry-Paraffin: Integrin alpha L/CD11a Antibody [NBP1-51967] - (3.8ug/ml) Staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Integrin alpha L/CD11a Antibody

Application

Recommended Usage

Immunohistochemistry

4 - 6 ug/ml

Immunohistochemistry-Paraffin

4-6 ug/ml

Peptide ELISA

1:16000

Western Blot

0.5-2 ug/ml

Application Notes

WB: Approx. 200 kDa band observed in human spleen and tonsil lysates (calculated MW of 129 kDa band according to NP_002200.2). The observed molecular weight corresponds to earlier findings with different antibodies from other sources. IHC-P: Human liver shows selected staining of cells within aggregated splenocytes of the white pulp.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Integrin alpha L/CD11a

CD11a is a 180 kD glycoprotein, also known as alpha-L integrin, LFA-1 alpha, Ly-15, or Ly-21. It is a member of the integrin family, primarily expressed on lymphocytes, monocytes/macrophages, and granulocytes. In association with CD18, the CD11a/CD18 complex forms LFA-1. CD11a plays an important role in intercellular adhesion and costimulation by binding its ligands, ICAM-1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50). The 2D7 antibody can block CD11a-mediated cellular adhesion.

Alternate Names

CD11a, ITGAL, LFA-1 alpha, Ly-15, Ly-21

Entrez Gene IDs

3683 (Human)

Gene Symbol

ITGAL

UniProt

NP_002200.2 (Human)

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Product Specific Notices for Integrin alpha L/CD11a Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.