Integrin alpha V beta 6 (aVb6) is a heterodimeric adhesion protein that is exclusively expressed on subsets of epithelial cells, especially during development, after injury or inflammation, or on many carcinomas. Active aVb6 binds to the extracellular matrix proteins Fibronectin and Tenascin C as well as the TGF-beta latency-associated peptide (LAP). It cooperates with LTBP-1, Fibronectin, and PAR-1 to activate TGF-beta 1 or TGF-beta 3 from large latent complexes. aVb6 also plays a role in Treg development, tumor progression, and virus cellular entry.
Integrin alpha V beta 6 Antibody (AvB6 53a.2) - BSA Free
Novus Biologicals | Catalog # NBP2-50449
![Immunoprecipitation: Integrin alpha V beta 6 Antibody (AvB6 53a.2) - BSA Free [NBP2-50449]](https://resources.rndsystems.com/images/products/Integrin-alpha-V-+-beta-6-Antibody-AvB6-53a-2-Western-Blot-NBP2-50449-img0002.jpg "Immunoprecipitation: Integrin alpha V beta 6 Antibody (AvB6 53a.2) - BSA Free [NBP2-50449]")
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Functional, CyTOF-ready
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # AvB6 53a.2
Format
BSA Free
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Product Specifications
Immunogen
Mouse fibroblasts retrovirally infected with cDNA to human Integrin alpha V beta 6 (3T3b6.19). Cells expressed a mouse/human Integrin alpha V beta 6 chimera.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Integrin alpha V beta 6 Antibody (AvB6 53a.2) - BSA Free
Immunoprecipitation: Integrin alpha V beta 6 Antibody (AvB6 53a.2) - BSA Free [NBP2-50449]
Immunoprecipitation: Integrin alpha V beta 6 Antibody (AvB6 53a.2) [NBP2-50449] - avb6 was immunoprecipitated with rat mAbs and western blotted for B6 with C19 antibody. Film was then exposed for different time exposures. The control lane was DX3puroB6 lysate. 59A, 10B2, 620W and 12D shows a very good result in revealing the double band for β6 by 5 seconds, and 53a2 shows only the upper band of B6. By 1 minute, most rat mAbs reveal a B6 immunoprecipitate. Data are from one of three separate experiments.Applications for Integrin alpha V beta 6 Antibody (AvB6 53a.2) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:10 - 1:1000
Immunocytochemistry/ Immunofluorescence
1:10 - 1:500
Immunoprecipitation
1:10 - 1:500
Application Notes
Positive control(s): BT20 breast carcinoma, original immunogen. This antibody is Cytof ready.
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Integrin alpha V beta 6
Alternate Names
CD51, integrin subunit alpha V, ITGAV, MSK8, VNRA, VTNR
Gene Symbol
ITGAV
Additional Integrin alpha V beta 6 Products
Product Documents for Integrin alpha V beta 6 Antibody (AvB6 53a.2) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Integrin alpha V beta 6 Antibody (AvB6 53a.2) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for Integrin alpha V beta 6 Antibody (AvB6 53a.2) - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunoprecipitation Protocol
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Liperfluo
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Integrin alpha V beta 6 Antibody (AvB6 53a.2) - BSA Free
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Q: Is your Integrin alpha V beta 6 Antibody (AvB6 53a.2) a mixed clone?
A: Yes, this monoclonal antibody contain two clones: clones AvB6 and 53a.2 which are against Integrin alpha V and Integrin beta 6, respectively. Clone 53a2 is against human αvβ6 (PMID: 18451156 ). Clone AvB6 was produced using immunogen "Mouse fibroblasts retrovirally infected with cDNA to human beta6 (3T3b6.19). The cells expressed a mouse/human avb6 chimera i.e. Integrin alpha V is from mouse, whereas beta 6 is from human.
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