Integrin alpha V/CD51 Antibody (SC56-07)

Name: Integrin alpha V/CD51 Antibody (SC56-07)
Brand: Novus Biologicals
SKU: NBP3-23955
Price: 449 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-23955

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # SC56-07

View all Integrin alpha V/CD51 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within of Human Integrin alpha V/CD51 aa 1000-1048 / 1048. (SwissProt: P06756 Human; SwissProt: P43406 Mouse)

Localization

Membrane.Membrane.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Integrin alpha V/CD51 Antibody (SC56-07)

Western Blot: Integrin alpha V/CD51 Antibody (SC56-07) [NBP3-23955] -

Western blot analysis of Integrin alpha V on A549 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours.

Immunocytochemistry/Immunofluorescence: Integrin alpha V/CD51 Antibody (SC56-07) [NBP3-23955] -

ICC staining of Integrin alpha V in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature.

Immunocytochemistry/Immunofluorescence: Integrin alpha V/CD51 Antibody (SC56-07) [NBP3-23955] -

ICC staining of Integrin alpha V in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature.

Flow Cytometry: Integrin alpha V/CD51 Antibody (SC56-07) [NBP3-23955] -

Analysis of Integrin alpha V was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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Applications for Integrin alpha V/CD51 Antibody (SC56-07)

Application

Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunohistochemistry-Paraffin

1:1000

Western Blot

1:1000-1:2000

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Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Integrin alpha V/CD51

Integrin alpha V chain interacts with the integrin beta 3 subunit/CD61 to form the alpha-V-beta-3 heterodimer/vitronectin receptor. It is expressed on endothelial cells, some activated leukocytes, NK cells, macrophages, neutrophils, and platelets. Integrin alpha V also forms heterodimers with the integrin beta 1, beta 5, beta 6, and beta 8 subunits. Alpha-V-beta-3 is an activation dependent receptor for platelet attachment and spreading on vitronectin and other matrix components.

Alternate Names

CD51, ITGAV, MSK8, Vitronectin Receptor alpha, VNRA

Gene Symbol

ITGAV

Additional Integrin alpha V/CD51 Products

Product Documents for Integrin alpha V/CD51 Antibody (SC56-07)

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Certificate of Analysis

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Product Specific Notices for Integrin alpha V/CD51 Antibody (SC56-07)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.