Integrin alpha V/CD51 Antibody (SC56-07)

Flow Cytometry: Integrin alpha V/CD51 Antibody (SC56-07) [NBP3-23955] -

Analysis of Integrin alpha V was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Western Blot: Integrin alpha V/CD51 Antibody (SC56-07) [NBP3-23955]

Western blot analysis on different lysates with NBP3-23955 at 1/5,000 dilution. Lane 1: A549 cell lysate Lane 2: HeLa cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 116 kDa Observed band size: 130 kDa Exposure time: 4 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-23955 at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.