Isopeptidase T/USP5 Antibody

Novus Biologicals | Catalog # NB100-77285

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Isopeptidase T/USP5 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 75 and 125 of human ubiquitin specific peptidase 5 (isopeptidase T) using the numbering given in entry CAA62690.1 (GeneID 8078).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

96 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Isopeptidase T/USP5 Antibody

Western Blot: Isopeptidase T/USP5 Antibody [NB100-77285]

Western Blot: Isopeptidase T/USP5 Antibody [NB100-77285]

Western Blot: Isopeptidase T/USP5 Antibody [NB100-77285] - Detection of Human and Mouse USP5/IsoT on HeLa whole cell lysate using NB100-77285. USP5/IsoT was also immunoprecipitated by rabbit anti-USP5/IsoT antibody NB100-77286.

Applications for Isopeptidase T/USP5 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Isopeptidase T/USP5

Ubiquitin (Ub) is a highly conserved protein found ubiquitously in eukaryotic organisms. The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific processing protease 5 (USP5) is a member of this family. The upregulation of USP5 has been found to be associated with fetal Down syndrome.

Long Name

Ubiquitin Specific Protease 5

Alternate Names

IsoT, USP5

Entrez Gene IDs

8078 (Human); 22225 (Mouse)

Gene Symbol

USP5

UniProt

P45974

Additional Isopeptidase T/USP5 Products

Product Documents for Isopeptidase T/USP5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Isopeptidase T/USP5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Deubiquitinating Enzymes (DUBs)

Citations for Isopeptidase T/USP5 Antibody

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