Janelia Fluor® 635, BromoCatch™ Ligand

Tocris Bioscience | Catalog # 8937

Fluorogenic Janelia Fluor® 635 conjugated covalent ligand for BromoCatch™, enabling no-wash live-cell imaging
Tocris Bioscience

Key Product Details

Description

Fluorogenic Janelia Fluor® 635 conjugated covalent ligand for BromoCatch™, enabling no-wash live-cell imaging

Wavelength

Excitation = 646 nm, Emission = 663 nm
View all BromoCatch Platform »

Product Description

Janelia Fluor® 635, BromoCatch™ Ligand is a fluorogenic BromoCatch™ ligand functionalized with Janelia Fluor® 635, designed for real-time, no-wash imaging of BromoCatch-tagged proteins in live cells. Upon covalent binding to the BromoCatch™ tag, this probe undergoes a fluorescence switch-on due to the environmental sensitivity of the Janelia Fluor® 635 dye, delivering bright, red-shifted signal with minimal background fluorescence. Janelia Fluor® 635, BromoCatch™ Ligand enables nuclear-targeted labeling of fusion constructs such as H2B-BromoCatch, and shows no off-target activity in wild-type or untransfected cells. Compatible with super-resolution imaging and suitable for dynamic cellular studies, Janelia Fluor® 635, BromoCatch™ Ligand offers a powerful tool for live-cell tracking of BromoCatch-tagged proteins with excellent photostability and imaging contrast.

Please refer to this protocol for further information on how to use this product.

Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.

This product is part of an early adopter program.

BromoCatch™ is a trademark of Bio-Techne Corp.

Scientific Data Images for Janelia Fluor® 635, BromoCatch™ Ligand

In gel fluorescence of Janelia Fluor® 635, BromoCatch Ligand​

In gel fluorescence of Janelia Fluor® 635, BromoCatch™ Ligand.

In gel assay titrating the different proteins with increasing concentrations of the Janelia Fluor® 635, BromoCatch™ Ligand​ probe.​
Multiplexed fluorescence imaging of HEK293 cells expressing GFP-BromoCatch™ and H2B-HaloTag®.

Multiplexed fluorescence imaging of HEK293 cells expressing GFP-BromoCatch™ and H2B-HaloTag®.

Cells were labeled with Hoechst 33342 (Cat# 5117), Janelia Fluor® 635, BromoCatch™ Ligand (Cat# 8937), (1µM), and Janelia Fluor® 549, Haloalkane (Cat# 8806), (100 nM). Intrinsic GFP fluorescence provides an orthogonal channel confirming GFP-BromoCatch™ expression, demonstrating robust multichannel compatibility in live-cell imaging.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus and the University of Dundee

Optical Data

Janelia Fluor<sup>®</sup> 635, BromoCatch™ Ligand Dye Spectra
λabs 646 nm
λem 663 nm
Cell Permeable Yes

Spectra Viewer

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Product Specifications for Janelia Fluor® 635, BromoCatch™ Ligand

Molecular Weight

1152.40

Formula

C61H67F2N9O8SSi

Storage

Store at -20°C

Purity

≥95% (HPLC)

Chemical Name

4-(((R)-14-((S)-4-(4-Acrylamidophenyl)-2,3,9-trimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepin-6-yl)-13-oxo-3,6,9-trioxa-12-azahexadecyl)carbamoyl)-2-(3-(3-fluoroazetidin-1-ium-1-ylidene)-7-(3-fluoroazetidin-1-yl)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)benzoate

InChI Key

KUWXUURGPXZQEI-RYOPHROTSA-N

SMILES

CC1=C(C)SC2=C1C(C3=CC=C(C=C3)NC(C=C)=O)=N[C@@H]([C@H](C(NCCOCCOCCOCCNC(C4=CC=C(C(C5=C6C([Si](C)(C7=CC(N8CC(C8)F)=CC=C57)C)=C/C(C=C6)=[N+]9CC(C/9)F)=C4)C([O-])=O)=O)=O)CC)C%10=NN=C(C)N2%10

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 11.52 10

Preparing Stock Solutions for Janelia Fluor® 635, BromoCatch™ Ligand

The following data is based on the product molecular weight 1152.40.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 8.68 mL 43.39 mL 86.78 mL
0.5 mM 1.74 mL 8.68 mL 17.36 mL
1 mM 0.87 mL 4.34 mL 8.68 mL
5 mM 0.17 mL 0.87 mL 1.74 mL

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Background References

References are publications that support the biological activity of the product.

Product Documents for Janelia Fluor® 635, BromoCatch™ Ligand

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Janelia Fluor® 635, BromoCatch™ Ligand

For research use only

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