Jarid1A Antibody (5B10P1)

Novus Biologicals | Catalog # NBP3-16478

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5B10P1 expressed in HEK293
View all Jarid1A Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1400-1690 of human Jarid1A (P29375). HAYSSASKSCSQGSSTPRKQPRKSPLVPRSLEPPVLELSPGAKAQLEELMMVGDLLEVSLDETQHIWRILQATHPPSEDRFLHIMEDDSMEEKPLKVKGKDSSEKKRKRKLEKVEQLFGEGKQKSKELKKMDKPRKKKLKLGADKSKELNKLAKKLAKEEERKKKKEKAAAAKVELVKESTEKKREKKVLDIPSKYDWSGAEESDDENAVCAAQNCQRPCKDKVDWVQCDGGCDEWFHQVCVGVSPEMAENEDYICINCAKKQGPVSPGPAPPPSFIMSYKLPMEDLKETS

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

192 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Jarid1A Antibody (5B10P1)

Western Blot: Jarid1A Antibody (5B10P1) [NBP3-16478]

Western Blot: Jarid1A Antibody (5B10P1) [NBP3-16478]

Western Blot: Jarid1A Antibody (5B10P1) [NBP3-16478] - Analysis of extracts of Mouse lung, using Jarid1A Rabbit mAb (NBP3-16478) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.
Western Blot: Jarid1A Antibody (5B10P1) [NBP3-16478]

Western Blot: Jarid1A Antibody (5B10P1) [NBP3-16478]

Western Blot: Jarid1A Antibody (5B10P1) [NBP3-16478] - Analysis of extracts of various cell lines, using Jarid1A Rabbit mAb (NBP3-16478) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunoprecipitation: Jarid1A Antibody (5B10P1) [NBP3-16478]

Immunoprecipitation: Jarid1A Antibody (5B10P1) [NBP3-16478]

Immunoprecipitation: Jarid1A Antibody (5B10P1) [NBP3-16478] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug Jarid1A antibody (NBP3-16478). Western blot was performed from the immunoprecipitate using Jarid1A antibody (NBP3-16478) at a dilition of 1:500.
Jarid1A Antibody (5B10P1)

Chromatin Immunoprecipitation: Jarid1A Antibody (5B10P1) [NBP3-16478] -

Chromatin Immunoprecipitation: Jarid1A Antibody (5B10P1) [NBP3-16478] - Analysis of extracts of HCT116 cells, using KDM5A antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Jarid1A Antibody (5B10P1)

Chromatin Immunoprecipitation: Jarid1A Antibody (5B10P1) [NBP3-16478] -

Chromatin Immunoprecipitation: Jarid1A Antibody (5B10P1) [NBP3-16478] - Chromatin immunoprecipitation analysis of extracts of HCT116 cells, using Jarid1A antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Jarid1A Antibody (5B10P1)

Chromatin Immunoprecipitation: Jarid1A Antibody (5B10P1) [Jarid1A] -

Chromatin Immunoprecipitation: Jarid1A Antibody (5B10P1) [Jarid1A] - Chromatin immunoprecipitation analysis of extracts of HCT116 cells, using Jarid1A antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Applications for Jarid1A Antibody (5B10P1)

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

10ug-15ug of Chromatin

Immunoprecipitation

-0.5μg-4μg antibody for 200μg-400μg extracts of whole cells

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Jarid1A

Jarid1A is encoded by this gene is a ubiquitously expressed nuclear protein. It binds directly, with several other proteins, to retinoblastoma protein which regulates cell proliferation. This protein also interacts with rhombotin-2 which functions distinctly in erythropoiesis and in T-cell leukemogenesis. Rhombotin-2 is thought to either directly affect the activity of the encoded protein or may indirectly modulate the functions of the retinoblastoma protein by binding to this protein. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Long Name

Lysine-specific demethylase 5A

Alternate Names

EC 1.14.11.67, KDM5A, RBBP-2, RBBP2, RBP2

Gene Symbol

KDM5A

Additional Jarid1A Products

Product Documents for Jarid1A Antibody (5B10P1)

Certificate of Analysis

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Product Specific Notices for Jarid1A Antibody (5B10P1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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