Jarid1A Antibody

Novus Biologicals | Catalog # NB110-40499

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Jarid1A Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1640 and 1690 of human JARID1A/RBP2 using the numbering given in entry NP_005047.1 (GeneID 5927).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

192 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Jarid1A Antibody

Western Blot: Jarid1A Antibody [NB110-40499]

Western Blot: Jarid1A Antibody [NB110-40499]

Western Blot: Jarid1A Antibody [NB110-40499] - Whole cell lysate (50 ug) from TCMK-1 and Renca cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-JARID1A/RBP2 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot: Jarid1A Antibody [NB110-40499]

Western Blot: Jarid1A Antibody [NB110-40499]

Western Blot: Jarid1A Antibody [NB110-40499] - Detection of Human and Mouse Jarid1A/RBP2 on HeLa whole cell lysate using NB110-40499. Jarid1A/RBP2 was also immunoprecipitated by other rabbit anti-Jarid1A/RBP2 antibodies.
Western Blot: Jarid1A Antibody [NB110-40499]

Western Blot: Jarid1A Antibody [NB110-40499]

Western Blot: Jarid1A Antibody [NB110-40499] - Detection of Human JARID1A/RBP2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-JARID1A/RBP2 antibody NB110-40499 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: Jarid1A Antibody [NB110-40499]

Immunoprecipitation: Jarid1A Antibody [NB110-40499]

Immunoprecipitation: Jarid1A Antibody [NB110-40499] - Detection of human JARID1A/RBP2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-JARID1A/RBP2 antibody NB110-40499 (lot NB110-40499-3) used for IP at 6 ug per reaction. JARID1A/RBP2 was also immunoprecipitated by a previous lot of this antibody (lot NB110-40499-2). For blotting immunoprecipitated JARID1A/RBP2, NB110-40499 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for Jarid1A Antibody

Application
Recommended Usage

Immunoprecipitation

4-10 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Jarid1A

The protein encoded by this gene is a ubiquitously expressed nuclear protein. It binds directly, with several other proteins, to retinoblastoma protein which regulates cell proliferation. This protein also interacts with rhombotin-2 which functions distinctly in erythropoiesis and in T-cell leukemogenesis. Rhombotin-2 is thought to either directly affect the activity of the encoded protein or may indirectly modulate the functions of the retinoblastoma protein by binding to this protein. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Long Name

Lysine-specific demethylase 5A

Alternate Names

EC 1.14.11.67, KDM5A, RBBP-2, RBBP2, RBP2

Entrez Gene IDs

5927 (Human); 214899 (Mouse)

Gene Symbol

KDM5A

UniProt

P29375 (Mouse)

Additional Jarid1A Products

Product Documents for Jarid1A Antibody

Certificate of Analysis

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Product Specific Notices for Jarid1A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Jarid1A Antibody

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