JMJD2B Antibody - BSA Free

Novus Biologicals | Catalog # NB100-74604

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Chicken

Cited:

Avian - Chicken

Applications

Validated:

Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Cited:

Western Blot, Chemotaxis

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all JMJD2B Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 625 and 675 of human jumonji domain containing 2B using the numbering given in entry NP_055830.1 (GeneID 23030).

Reactivity Notes

Chicken reactivity reported in scientific literature (PMID: 26598618)

Localization

Nuclear

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for JMJD2B Antibody - BSA Free

Immunoprecipitation: JMJD2B Antibody [NB100-74604]

Immunoprecipitation: JMJD2B Antibody [NB100-74604]

Immunoprecipitation: JMJD2B Antibody [NB100-74604] - Detection of human JMJD2B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-JMJD2B antibody NB100-74604 used for IP at 3 ug per reaction. JMJD2B was also immunoprecipitated by rabbit anti-JMJD2B antibody NB100-74605. For blotting immunoprecipitated JMJD2B, NB100-74604 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for JMJD2B Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

Immunoprecipitation

2-5 ug/mg lysate
Application Notes
Use in Western blot, and chromatin immunoprecipitation reported in scientific literature (PMID 26598618).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: JMJD2B

JMJD2B is a member of the jumonji (jmj) domain containing gene family of histone demethylases that plays a role in chromatin regulation and influences transcriptional activation and suppression. JMJD2B has been shown to be capable of antagonizing H3K9 trimethylation.

Long Name

Lysine-specific demethylase 4B

Alternate Names

EC 1.14.11.66, JHDM3B, KDM4B, KIAA0876

Entrez Gene IDs

23030 (Human)

Gene Symbol

KDM4B

UniProt

NP_055830.1 (Human)

Additional JMJD2B Products

Product Documents for JMJD2B Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for JMJD2B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for JMJD2B Antibody - BSA Free

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