JMJD2B Antibody - BSA Free

Western Blot: JMJD2B Antibody [NB100-74605]

Western Blot: JMJD2B Antibody [NB100-74605] - Detection of Human JMJD2B by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-JMJD2B antibody NB100-74605 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoprecipitation: JMJD2B Antibody [NB100-74605]

Immunoprecipitation: JMJD2B Antibody [NB100-74605] - Detection of human JMJD2B by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-JMJD2B antibody NB100-74605 (lot NB100-74605-2) used for IP at 6 ug/mg lysate. JMJD2B was also immunoprecipitated by a previous lot (lot NB100-74605-1) of this antibody For blotting immunoprecipitated JMJD2B, NB100-74605 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Western Blot: JMJD2B Antibody [NB100-74605]

JMJD2B-Antibody-Knockdown-Validated-NB100-74605-img0008.jpg

Western Blot: JMJD2B Antibody [NB100-74605] -

Western Blot: JMJD2B Antibody [NB100-74605] - Gomisin N (GN) represses the expression of JMJD2B, PPAR gamma 2, & PPAR gamma 2 steatosis target genes in HepG2 cells. HepG2 cells were treated with GN (100 μM) for 12 h. (A) JMJD2B expression was assessed by qPCR & western blotting. The full-length western blots corresponding to truncated blots are given in Supplementary Figure S4. (B) PPAR gamma 2 expression was measured by qPCR. (C,D) The expression of PPAR gamma 2 steatosis target genes was assessed by qPCR. Data represent means ± SEM of three independent experiments performed in triplicate. *p < 0.05 vs. no treatment. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30214048), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: JMJD2B Antibody [NB100-74605] -

Western Blot: JMJD2B Antibody [NB100-74605] - Overexpression of JMJD2B increases intracellular TG levels & stimulates PPAR gamma 2 expression in HepG2 cells. HepG2 cells in 6-well plates were infected with Ad-GFP or Ad-JMJD2B. (A) The levels of JMJD2B, H3K9me, H3K9me2, & H3K9me3 were measured by western blotting. The full-length western blots corresponding to truncated blots are provided in Supplementary Figure S2A. (B) Intracellular TG levels were measured by TG assay kit. (C) PPAR gamma 2 expression was assessed by qPCR & western blotting. The full-length western blots corresponding to truncated blots are presented in Supplementary Figure S2B. (D,E) The expression of PPAR gamma 2 steatosis target genes was evaluated by qPCR. Data represent means ± SEM of three independent experiments performed in triplicate. *p < 0.05, **p < 0.01 vs. Ad-GFP. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30214048), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: JMJD2B Antibody [NB100-74605] -

Western Blot: JMJD2B Antibody [NB100-74605] - JMJD2B expression increases in hepatic steatotic cell & animal models. (A) HepG2 cells were incubated with a mixture of palmitic acid (PA) & oleic acid (OA) (1:2 ratio) at 800 μM concentrations for 24 h, & intracellular triglyceride (TG) levels were analyzed by a TG assay kit. JMJD2B mRNA & protein levels were examined by qPCR & western blotting, respectively. Data represent means ± SEM of three independent experiments performed in triplicate. *p < 0.05 vs. no treatment. The full-length western blots corresponding to truncated blots are presented in Supplementary Figure S1A. (B) HepG2 cells were treated with T0901317 (10 μM) for 24 h, & intracellular triglyceride (TG) levels were measured by a TG assay kit. JMJD2B mRNA & protein levels were examined by qPCR & western blotting, respectively. Data represent means ± SEM of three independent experiments performed in triplicate. *p < 0.05 vs. no treatment. The full-length western blots are presented in Supplementary Figure S1B. (C) Total RNAs were isolated from the livers of HFD-induced obese mice. The JMJD2B mRNA levels were assessed by qPCR. Data represent means ± SEM of 5 mice. *p < 0.05 vs. ND mice. ND: normal diet. HFD: high fat diet. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30214048), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: JMJD2B Antibody [NB100-74605] -

Western Blot: JMJD2B Antibody [NB100-74605] - Knockdown of JMJD2B reduces the intracellular TG level & represses PPAR gamma 2 expression in HepG2 cells. HepG2 cells in 6-well plates were transfected with scramble RNA or JMJD2B siRNA. (A) The levels of JMJD2B, H3K9me, H3K9me2, & H3K9me3 were measured by western blotting. The full-length western blots corresponding to truncated blots are presented in Supplementary Figure S3A. (B) Intracellular TG levels were measured by a TG assay kit. (C) PPAR gamma 2 expression was assessed by qPCR & western blotting. The full-length western blots corresponding to truncated blots are presented in Supplementary Figure S3B. (D,E) The expression of PPAR gamma 2 steatosis target genes was evaluated by qPCR. Data represent means ± SEM of three independent experiments performed in triplicate. *p < 0.05 vs. scramble RNA. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30214048), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: JMJD2B Antibody [NB100-74605] -

Western Blot: JMJD2B Antibody [NB100-74605] - JMJD2B expression increases in hepatic steatotic cell & animal models. (A) HepG2 cells were incubated with a mixture of palmitic acid (PA) & oleic acid (OA) (1:2 ratio) at 800 μM concentrations for 24 h, & intracellular triglyceride (TG) levels were analyzed by a TG assay kit. JMJD2B mRNA & protein levels were examined by qPCR & western blotting, respectively. Data represent means ± SEM of three independent experiments performed in triplicate. *p < 0.05 vs. no treatment. The full-length western blots corresponding to truncated blots are presented in Supplementary Figure S1A. (B) HepG2 cells were treated with T0901317 (10 μM) for 24 h, & intracellular triglyceride (TG) levels were measured by a TG assay kit. JMJD2B mRNA & protein levels were examined by qPCR & western blotting, respectively. Data represent means ± SEM of three independent experiments performed in triplicate. *p < 0.05 vs. no treatment. The full-length western blots are presented in Supplementary Figure S1B. (C) Total RNAs were isolated from the livers of HFD-induced obese mice. The JMJD2B mRNA levels were assessed by qPCR. Data represent means ± SEM of 5 mice. *p < 0.05 vs. ND mice. ND: normal diet. HFD: high fat diet. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30214048), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: JMJD2B Antibody [NB100-74605] -

Western Blot: JMJD2B Antibody [NB100-74605] - Overexpression of JMJD2B increases intracellular TG levels & stimulates PPAR gamma 2 expression in HepG2 cells. HepG2 cells in 6-well plates were infected with Ad-GFP or Ad-JMJD2B. (A) The levels of JMJD2B, H3K9me, H3K9me2, & H3K9me3 were measured by western blotting. The full-length western blots corresponding to truncated blots are provided in Supplementary Figure S2A. (B) Intracellular TG levels were measured by TG assay kit. (C) PPAR gamma 2 expression was assessed by qPCR & western blotting. The full-length western blots corresponding to truncated blots are presented in Supplementary Figure S2B. (D,E) The expression of PPAR gamma 2 steatosis target genes was evaluated by qPCR. Data represent means ± SEM of three independent experiments performed in triplicate. *p < 0.05, **p < 0.01 vs. Ad-GFP. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30214048), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: JMJD2B Antibody [NB100-74605] -

Immunohistochemistry: JMJD2B Antibody [NB100-74605] - Adenovirus-mediated JMJD2B overexpression stimulated hepatic steatosis in vivo. C57BL/6 mice (8 weeks old) were injected with adenovirus Ad-GFP or Ad-JMJD2B. After injection, Ad-injected mice were fed a HFD for 2 weeks. (A) JMJD2B expression was determined by qPCR (B) Immunostaining of JMJD2B (scale bar = 50 μm). Representative photographs are shown. JMJD2B positive cells were counted in ten random areas at 400× magnification & analyzed by using image J. Quantification of immunostaining assay is represented as percentage of JMJD2B positive cells. (C) Hepatic triglyceride (TG) levels were measured by a TG assay kit. (D) Liver morphology, ORO & H&E staining (scale bar = 100 μm). Representative images are presented. (E) Serum total TG levels. (F) Serum total cholesterol levels. Data represent means ± SEM from 5 mice. *p < 0.05 vs. Ad-GFP-infected mice. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30214048), licensed under a CC-BY license. Not internally tested by Novus Biologicals.