Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Primate (92%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), ICC/IF (Negative)

Cited:

Western Blot, Chemotaxis

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all JMJD2D Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to a C-terminal portion of the human JMJD2D protein (within residues 460-520). [Swiss-Prot# Q6B0I6]

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: monkey (92%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for JMJD2D Antibody - BSA Free

Western Blot: JMJD2D AntibodyBSA Free [NBP1-03357]

Western Blot: JMJD2D AntibodyBSA Free [NBP1-03357]

Western Blot: JMJD2D Antibody [NBP1-03357] - Western Blot Image of anti-JMJD2D. Whole cell protein from PC3 (lane 1), SHSY5Y (lane 2), and HeLa (lane 3) was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-JMJD2D in 1% milk, and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Applications for JMJD2D Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation

reported in scientific literature

Western Blot

0.2 ug/ml
Application Notes
This JMJD2D antibody is useful for Western blot, where a band is seen at ~55 kDa. This antibody is not useful for ICC/IF. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reviewed Applications

Read 2 reviews rated 5 using NBP1-03357 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: JMJD2D

Jumonji lysine demethylases (JMJDs) are oxygenases catalyzing the demethylation of mono-, di-, or trimethylated lysines, and its members (JMJD2A, JMJD2B, JMJD2C, and JMJD2D/ KDM4D) are Fe(II)-/alpha-ketoglutarate-dependent enzymes harboring a JmjC catalytic domain. JMJD2 shows differential substrate specificities and unlike, JMJD2A, JMJD2B, and JMJD2C that efficiently demethylate H3K9me3 as well as H3K36me3, JMJD2D is specific for H3K9me3 site. These enzymes have been implicated in transcriptional regulation, cell-cycle progression, nuclear hormone signaling, embryonic stem cell self-renewal and development. Overexpression of several JMJD2 homologs has been linked to cancer e.g. JMJD2D, JMJD2A and JMJD2C are overexpressed in prostate cancer wherein they function as AR coactivators to upregulate AR target genes and JMJD2D has been shown to function as a pro-proliferative protein in colon cancer cells. JMJD2D has also been reported to interact with the ligand binding domain of AR and thereby activate AR gene transcription.

Long Name

Lysine-specific demethylase 4D

Alternate Names

JHDM3D, KDM4D

Entrez Gene IDs

55693 (Human)

Gene Symbol

KDM4D

UniProt

Q6B0I6 (Human)

Additional JMJD2D Products

Product Documents for JMJD2D Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for JMJD2D Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for JMJD2D Antibody - BSA Free

Customer Reviews for JMJD2D Antibody - BSA Free (2)

5 out of 5
2 Customer Ratings
5 Stars
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4 Stars
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3 Stars
0%
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1 Stars
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Showing  1 - 2 of 2 reviews Showing All
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  • JMJD2D Antibody
    Name: ramzi abbassi
    Application: Western Blot
    Sample Tested: glioblastoma cell lysates and primary cancer cells
    Species: Human
    Verified Customer | Posted 03/25/2019
    25 ug of lysate loaded per well (0.8 ug/uL) of naive vs treated FPW1 primary glioblastoma cells. Three independent repeat-pairs are shown. Exposure of about 1 min.
    Bolt system (Life Technologies) was used. Prior to running the gel, DTT was added to the samples and samples were heated for 5 min at 95 degrees Celsius and rapidly cooled on ice.
    JMJD2D Antibody - BSA Free NBP1-03357
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: MDA-MB-435 cell lysates
    Species: Human
    Verified Customer | Posted 01/11/2012
    JMJD2D Antibody - BSA Free NBP1-03357

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Protocols

View specific protocols for JMJD2D Antibody - BSA Free (NBP1-03357):

JMJD2D Antibody:
Western Blot Protocol

1. Perform SDS-PAGE (4-12% MOPS) on samples to be analyzed, loading 30 ug of total protein per lane.
2. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer
apparatus.
3. Rinse membrane with dH2O and then stain the blot using Ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
4. Rinse the blot in TBS for approximately 5 minutes.
5. Block the membrane using 5% BSA in TBS + Tween, 1 hour at RT.
6. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each.
7. Dilute the rabbit anti-JMJD2D primary antibody (NBP1-03357) in blocking buffer and incubate 1 hour at room temperature.
8. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each.
9. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturers
instructions) and incubate 1 hour at room temperature.
10. Wash the blot in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce ECL).

Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2%, provided it does not interfere with antibody-antigen binding.

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