Kallikrein 11 Antibody (121) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-89630
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Western Blot, ELISA, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 121 expressed in HEK293
Format
Azide and BSA Free
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Product Specifications
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Human Kallikrein 11 (Met 1-Asn 250; Accession#: NP_006844.1).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for Kallikrein 11 Antibody (121) - Azide and BSA Free
Western Blot: Kallikrein 11 Antibody (121) [NBP2-89630]
Western Blot: Kallikrein 11 Antibody (121) [NBP2-89630] - Lane A: A549 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:31 kDa Observed band size:42 kDaImmunoprecipitation: Kallikrein 11 Antibody (121) [NBP2-89630]
Immunoprecipitation: Kallikrein 11 Antibody (121) [NBP2-89630] - Lane A:0.5 mg Hela Whole Cell Lysate 1 uL anti-KLK11 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-KLK11 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 31 kDa Observed band size: 49 kDaApplications for Kallikrein 11 Antibody (121) - Azide and BSA Free
Application
Recommended Usage
ELISA
1:25000-1:50000
Immunoprecipitation
0.2-1 uL/mg of lysate
Western Blot
1:500-1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Kallikrein 11
Alternate Names
KLK11
Gene Symbol
KLK11
Additional Kallikrein 11 Products
Product Documents for Kallikrein 11 Antibody (121) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Kallikrein 11 Antibody (121) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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