Kappa Light Chain Antibody (RM103)

Novus Biologicals | Catalog # NBP2-62003

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM103 expressed in HEK293
View all Kappa Light Chain Primary Antibodies »

Product Specifications

Immunogen

Mouse IgG

Specificity

This antibody reacts to the kappa light chain of mouse immunoglobulins. No cross reactivity with the lambda light chain, human IgG, rat IgG, or goat IgG.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Kappa Light Chain Antibody (RM103)

Western Blot: Kappa Light Chain Antibody (RM103) [Unconjugated] [NBP2-62003] - Western blot of nonreduced(-) and reduced(+) mouse IgG1 kappa and IgG1 lambda, using NBP2-62003. This antibody reacts to nonreduced IgG1k (~150 kDa), and slightly reacts to reduced kappa light chain (~25 kDa).
Kappa Light Chain Antibody (RM103)

ELISA: Kappa Light Chain Antibody (RM103) - Azide and BSA Free [NBP2-62003] -

ELISA: Kappa Light Chain Antibody (RM103) - Azide and BSA Free [NBP2-62003] - A titer ELISA of mouse IgG1k. The plate was coated with different amounts of mouse IgG1k. A serial dilution of RM103 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Kappa Light Chain Antibody (RM103)

ELISA: Kappa Light Chain Antibody (RM103) - Azide and BSA Free [NBP2-62003] -

ELISA: Kappa Light Chain Antibody (RM103) - Azide and BSA Free [NBP2-62003] - ELISA of mouse immunoglobulins shows RM103 reacts to the kappa light chain of mouse immunoglobulins. No cross reactivity with the lamda light chain, human IgG (H + L), rat IgG (H + L), or goat IgG (H + L). The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM103 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

Applications for Kappa Light Chain Antibody (RM103)

Application
Recommended Usage

ELISA

0.005ug/ml-0.2ug/ml

Western Blot

0.1ug/ml-0.5ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50% Glycerol/PBS, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Kappa Light Chain

Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non-Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin-like domain. The EU sequence has the INV allotypic marker, Ala-45 and Val-83. The ROY sequence has the INV allotypic marker, Ala-45 and Leu-83.

Alternate Names

HCAK1, IGKCD, immunoglobulin kappa constant, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809

Gene Symbol

IGKC

Additional Kappa Light Chain Products

Product Documents for Kappa Light Chain Antibody (RM103)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Kappa Light Chain Antibody (RM103)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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