Kidney Matched Primary Tumor and Metastatic Tumor Slides - Frozen
Novus Biologicals | Catalog # NBP2-77664
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Key Product Details
Species
Human
Applications
Functional Assay, Immunohistochemistry
Product Summary for Kidney Matched Primary Tumor and Metastatic Tumor Slides - Frozen
Frozen tissue slides are ideal for rapid cellular localization of RNA and protein expression, especially for antibodies that don't recognize paraffin embedded tissue.
Tissues were snap frozen in liquid nitrogen immediately after excision, and embedded in OCT. Frozen tissue sections in each pair are prepared from the same donor. Tissue sections are 5-10 m in thickness, mounted on positively charged glass slides, and fixed by cold acetone. At least one of the tissue slides from each lot was stained with H & E for quality assurance. Product is supplied as 5 diseased slides and 5 adjacent normal slides.
Tissues were snap frozen in liquid nitrogen immediately after excision, and embedded in OCT. Frozen tissue sections in each pair are prepared from the same donor. Tissue sections are 5-10 m in thickness, mounted on positively charged glass slides, and fixed by cold acetone. At least one of the tissue slides from each lot was stained with H & E for quality assurance. Product is supplied as 5 diseased slides and 5 adjacent normal slides.
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Product Specifications
Application Notes
Each individual tissue spot should be >90% core retention and total core lost from 1 slide should be no more than 5%
Type
Matched Primary Tumor and Metastatic Tumor
Tissue
Kidney
Formulation, Preparation, and Storage
Concentration
Concentration is not relevant for this product. Please see the protocols for proper use of this product.
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at -70C. Avoid freeze-thaw cycles.
Product Documents for Kidney Matched Primary Tumor and Metastatic Tumor Slides - Frozen
Product Specific Notices for Kidney Matched Primary Tumor and Metastatic Tumor Slides - Frozen
Donor information available upon request
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Slides are guaranteed for 3 months from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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