KIF1C Antibody

Novus Biologicals | Catalog # NB100-57510

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all KIF1C Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1053 and the C-terminus (residue 1103) of human kinesin family member 1C (lethal toxin sensitivity 1) using the numbering given in entry NP_006603.2 (GeneID 10749).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for KIF1C Antibody

Western Blot: KIF1C Antibody [NB100-57510]

Western Blot: KIF1C Antibody [NB100-57510]

KIF1C-Antibody-Western-Blot-NB100-57510-img0002.jpg
Immunoprecipitation: KIF1C Antibody [NB100-57510]

Immunoprecipitation: KIF1C Antibody [NB100-57510]

Immunoprecipitation: KIF1C Antibody [NB100-57510] - Detection of Human and Mouse KIF1C/LTXS1 on HeLa whole cell lysate using NB100-57510. KIF1C/LTXS1 was also IPed by rabbit anti-KIF1C/LTXS1 antibodies NB100-57507, NB100-57508 and NB100-57509.
KIF1C Antibody

Western Blot: KIF1C Antibody [NB100-57510] -

KIF1C is a novel HOOK3-interacting protein.sfGFP-3×FLAG and full length (FL) HOOK3, HOOK3 (1–552), and HOOK3 (553–718) all tagged with sfGFP and 3×FLAG were immunoprecipitated with alpha -FLAG antibodies from transiently transfected HEK-293T cells. Western blots with alpha -HC, alpha -FAM160A2, alpha -KIF1C, and alpha -FLAG antibodies were used to determine which proteins co-immunoprecipitated with each HOOK3 construct.DOI:https://dx.doi.org/10.7554/eLife.28257.015

Applications for KIF1C Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 5 ug/mg lysate

Western Blot

1:2000-1:10000

Reviewed Applications

Read 1 review rated 1 using NB100-57510 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: KIF1C

The kinesin superfamily of proteins consists of over forty KIF motor proteins that function in intracellular transport along microtubules. Kinesin activity has been linked to various cellular functions such as vesicle transport, mitotic spindle formation, chromosome segregation, and cytokinesis. Structurally, all kinesins contain a motor domain with microtubule and nucleotide binding sites that utilize ATP to target cargo along microtubule filaments. KIF1C, also known as LTXS1 (lethal toxin sensitivity), has been found to be localized to the golgi and the endoplasmic reticulum (ER) and play a role in vesicle transport between the golgi and ER. Alternate identifiers of KIF1C include kinesin family member 1C, kinesin-like protein KIF1C and KIAA0706.

Alternate Names

KIAA0706, kinesin family member 1C, kinesin-like protein KIF1C, LTXS1

Entrez Gene IDs

10749 (Human); 16562 (Mouse)

Gene Symbol

KIF1C

UniProt

NP_006603.2 (Human)

Additional KIF1C Products

Product Documents for KIF1C Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for KIF1C Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for KIF1C Antibody (1)

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  • KIF1C Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: isolated adult cardiomyocytes
    Species: Mouse
    Verified Customer | Posted 12/08/2016
    Review was tested in an untested application, ICC, for which the customer earned a voucher for 100% the purchase price of the product as part of our Innovator's Reward Program.
    Tried both PFA and methanol fixation. Incubated primary at dilutions of 1:50 and 1:100, and incubated at room temp for 3 hours and at 4C overnight- all different conditions gave no signal using a alexa fluor 488 secondary.
    KIF1C Antibody NB100-57510

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