KIFAP3 Antibody (28E9) - BSA Free

Immunohistochemistry: KIFAP3 Antibody (28E9) [NBP3-26181] -

Immunohistochemistry: KIFAP3 Antibody (28E9) [NBP3-26181] - Image of KIFAP3 Antibody (28E9) diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Western Blot: KIFAP3 Antibody (28E9) [NBP3-26181] -

Western Blot: KIFAP3 Antibody (28E9) [NBP3-26181] - Positive Western Blot detected in: Hela whole cell lysate.
All lanes: KIFAP3 Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 92, 87, 83 kDa
Observed band size: 72-95 kDa

Immunocytochemistry/Immunofluorescence: KIFAP3 Antibody (28E9) [NBP3-26181] -

Immunocytochemistry/Immunofluorescence: KIFAP3 Antibody (28E9) [NBP3-26181] - Staining of PC-3 cell with KIFAP3 Antibody (28E9) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 552-conjugated Goat Anti-Rabbit IgG (H+L).