KIR2DL1/CD158a Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-99894

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all KIR2DL1/CD158a Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human KIR2DL1/CD158a (Accession#: NP_055033.2; Met1-His245)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Applications for KIR2DL1/CD158a Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

1:5000-1:10000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: KIR2DL1/CD158a

KIRDL2, also known as CD158a, belongs to the killer-cell immunoglobulin-like receptor (KIR) gene family expressed by natural killer (NK) cells and functions in inhibitory signaling (1,2). The KIR genes consisting of 15 gene loci and two pseudogenes are located on chromosome 19 and segregated into halptype A or B depending on gene composition (1). Human KIR2DL1 belongs to haplotype A and is synthesized as a protein of 348 amino acids (aa) in length with a theoretical molecular weight (MW) of 38.5 kDa (3). The KIR2DL1 protein consists of two extracellular immunoglobulin (Ig)-like C2 domains, a transmembrane domain, and a long cytoplasmic tail containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) (1,3). KIR2DL1 binds human leukocyte antigen (HLA) alleles with the C2 supratype, resulting in an inhibitory immune response and suppression of NK cell activity to target cells (1,2). KIR2DL1 has been shown to be a potential clinical biomarker for diseases related to suppressed NK cell function (4). An increase in inhibitory receptors such as KIR2DL1/CD158a is observed in certain pathologies like endometriosis and multiple myeloma (4,5). Therapeutic inhibition of KIR2DL1 may be a potential treatment option for endometriosis patients (5). Additionally, blocking of KIR2DL1 combined with rituximab, and anti-CD20 monoclonal antibody, has shown increased NK cell cytotoxicity than just the antibody itself (5).

References

1. Debska-Zielkowska J, Moszkowska G, Zielinski M, et al. KIR Receptors as Key Regulators of NK Cells Activity in Health and Disease. Cells. 2021;10(7):1777. Published 2021 Jul 14. https://doi.org/10.3390/cells10071777

2. Goodson-Gregg FJ, Krepel SA, Anderson SK. Tuning of human NK cells by endogenous HLA-C expression. Immunogenetics. 2020;72(4):205-215. https://doi.org/10.1007/s00251-020-01161-x

3. Uniprotkb (P43626)

4. Konjevic G, Jurisic V, Jovic V, et al. Investigation of NK cell function and their modulation in different malignancies. Immunol Res. 2012;52(1-2):139-156. https://doi.org/10.1007/s12026-012-8285-7

5. Hoogstad-van Evert J, Paap R, Nap A, van der Molen R. The Promises of Natural Killer Cell Therapy in Endometriosis. Int J Mol Sci. 2022;23(10):5539. Published 2022 May 16. https://doi.org/10.3390/ijms23105539

Long Name

Killer Cell Immunoglobulin-like Receptor, Two Domain Long Cytoplasmic Tail, 1

Alternate Names

CD158a

Gene Symbol

KIR2DL1

Additional KIR2DL1/CD158a Products

Product Documents for KIR2DL1/CD158a Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for KIR2DL1/CD158a Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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