Kir6.1 Overexpression Lysate

Novus Biologicals | Catalog # NBL1-12174

Novus Biologicals
100% Guarantee

Key Product Details

Species

Human

Applications

Western Blot

Product Summary for Kir6.1 Overexpression Lysate

Kir6.1 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC200340

Accession#: NM_004982

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.

Product Specifications

Application Notes

This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.

Theoretical MW

47.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Type

Overexpression

Scientific Data Images for Kir6.1 Overexpression Lysate

Western Blot: Kir6.1 Overexpression Lysate [NBL1-12174]

Western Blot: Kir6.1 Overexpression Lysate [NBL1-12174]

Western Blot: Kir6.1 Overexpression Lysate (Native) [NBL1-12174] - 1. Negative control, 2. HEK cells overexpressing Kir6.1. 15ug lysate each was loaded onto a 10% SDS-polyacrylamide gel and transferred to a PVDF membrane, and incubated with an anti-Kir6.1 antibody. Image from verified customer review.
Western Blot: Kir6.1 Overexpression Lysate [NBL1-12174]

Western Blot: Kir6.1 Overexpression Lysate [NBL1-12174]

Western Blot: Kir6.1 Overexpression Lysate (Adult Normal) [NBL1-12174] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Kir6.1.

Formulation, Preparation, and Storage

Formulation

RIPA buffer

Concentration

The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: Kir6.1

Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which has a greater tendency to allow potassium to flow into a cell rather than out of a cell, is controlled by G-proteins. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Long Name

ATP-sensitive inward rectifier potassium channel 8

Alternate Names

KCNJ8, uKATP-1

Gene Symbol

KCNJ8

Additional Kir6.1 Products

Product Documents for Kir6.1 Overexpression Lysate

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Kir6.1 Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Citations for Kir6.1 Overexpression Lysate

Customer Reviews for Kir6.1 Overexpression Lysate (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Kir6.1 Overexpression Lysate?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Kir6.1 Overexpression Lysate (Native)
    Name: Anonymous
    Verified Customer | Posted 05/08/2018
    1. Negative control 2. HEK cells overexpressing Kir6.1 Western blot where 15µg of each lysate was loaded onto a 10% SDS-polyacrylamide gel and transferred to a PVDF membrane, later incubated with an anti-Kir6.1 antibody.
    Kir6.1 Overexpression Lysate NBL1-12174

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Lysates