Kirrel2/NEPH3 Antibody (2B9-1D3) - Azide and BSA Free

Novus Biologicals | Catalog # H00084063-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2B9-1D3

Format

Azide and BSA Free
View all Kirrel2/NEPH3 Primary Antibodies »

Product Specifications

Immunogen

KIRREL2 (AAH07312, 1 a.a. ~ 219 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MLRMRVPALLVLLFCFRGRAGPSPHLLQQPEDLVVLLGEGGAQASLGRRASASFSEQKNLMRIPGSSDGSSSRGPEEEETGSREDRGPIVHTDHSDLVLEEEGTLETKDPTNGYYKVRGVSVSLSLGEAPGGGLFLPPPSPLGPPGTPTFYDFNPHLGMVPPCRLYRARAGYLTTPHPRAFTSYIKPTSFGPPDLAPGTPPFPYAAFPTPSHPRLQTHV

Reactivity Notes

Human. Other species not tested.

Specificity

KIRREL2 - kin of IRRE like 2 (Drosophila)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for Kirrel2/NEPH3 Antibody (2B9-1D3) - Azide and BSA Free

Western Blot: Kirrel2/NEPH3 Antibody (2B9-1D3) [H00084063-M01]

Western Blot: Kirrel2/NEPH3 Antibody (2B9-1D3) [H00084063-M01]

Western Blot: Kirrel2/NEPH3 Antibody (2B9-1D3) [H00084063-M01] - KIRREL2 monoclonal antibody (M01), clone 2B9-1D3 Analysis of KIRREL2 expression in Hela S3 NE.
Western Blot: Kirrel2/NEPH3 Antibody (2B9-1D3) [H00084063-M01]

Western Blot: Kirrel2/NEPH3 Antibody (2B9-1D3) [H00084063-M01]

Western Blot: Kirrel2/NEPH3 Antibody (2B9-1D3) [H00084063-M01] - Analysis of KIRREL2 expression in transfected 293T cell line by KIRREL2 monoclonal antibody (M01), clone 2B9-1D3.Lane 1: KIRREL2 transfected lysate (Predicted MW: 23.6 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation: Kirrel2/NEPH3 Antibody (2B9-1D3) [H00084063-M01]

Immunoprecipitation: Kirrel2/NEPH3 Antibody (2B9-1D3) [H00084063-M01]

Immunoprecipitation: Kirrel2/NEPH3 Antibody (2B9-1D3) [H00084063-M01] - Analysis of KIRREL2 transfected lysate using anti-KIRREL2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with KIRREL2 MaxPab rabbit polyclonal antibody.
Sandwich ELISA: Kirrel2/NEPH3 Antibody (2B9-1D3) [H00084063-M01] - Detection limit for recombinant GST tagged KIRREL2 is approximately 0.3ng/ml as a capture antibody.

Applications for Kirrel2/NEPH3 Antibody (2B9-1D3) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Kirrel2/NEPH3

Kirrel (kin of irregular chiasm-1-like) proteins, also known as the NEPH (nephrin-like) family, are type I transmembrane glycoproteins within the immunoglobulin superfamily. Kirrel1 (NEPH1), Kirrel2 (NEPH3, Filtrin, NLG-1) and Kirrel3 (NEPH2, kirre) share 34 - 44% amino acid (aa) identity, and all interact with nephrin, podocin and the adaptor molecule ZO-1 (1, 4). All can be found in podocytes of the kidney glomerulus, and play a role in barrier function in the slit diaphragm. (2-6). Kirrel1 can also be found in central nervous system neurons, pancreas and placenta, Kirrel2 in pancreatic islet beta cells, brain and lymph nodes, and Kirrel3 in the brain and bone marrow, typically at sites where nephrin is also expressed.

Long Name

Kin of Irregular Chiasm-like 2, Splice Variant A

Alternate Names

Filtrin, NEPH3, NLG1

Entrez Gene IDs

84063 (Human)

Gene Symbol

KIRREL2

OMIM

607762 (Human)

UniProt

AAH07312 (Human)

Additional Kirrel2/NEPH3 Products

Product Documents for Kirrel2/NEPH3 Antibody (2B9-1D3) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Kirrel2/NEPH3 Antibody (2B9-1D3) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Immunoglobulin Superfamily CAMs

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