KLC1 Antibody (5C3P5)

Novus Biologicals | Catalog # NBP3-15712

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5C3P5 expressed in HEK293
View all KLC1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 450-550 of human KLC1 (Q07866). GGWYKACKVDSPTVTTTLKNLGALYRRQGKFEAAETLEEAAMRSRKQGLDNVHKQRVAEVLNDPENMEKRRSRESLNVDVVKYESGPDGGEEVSMSVEWNG

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for KLC1 Antibody (5C3P5)

Western Blot: KLC1 Antibody (5C3P5) [NBP3-15712]

Western Blot: KLC1 Antibody (5C3P5) [NBP3-15712]

Western Blot: KLC1 Antibody (5C3P5) [NBP3-15712] - Western blot analysis of extracts of various cell lines, using Kinesin light chain 1 (KLC1) antibody (NBP3-15712) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Western Blot: KLC1 Antibody (5C3P5) [NBP3-15712]

Western Blot: KLC1 Antibody (5C3P5) [NBP3-15712]

Western Blot: KLC1 Antibody (5C3P5) [NBP3-15712] - Western blot analysis of extracts of Mouse brain, using Kinesin light chain 1 (KLC1) antibody (NBP3-15712) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Applications for KLC1 Antibody (5C3P5)

Application
Recommended Usage

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: KLC1

Conventional kinesin is a tetrameric molecule composed of two heavy chains and two light chains, and transports various cargos along microtubules toward their plus ends. The heavy chains provide the motor activity, while the light chains bind to various cargos. This gene encodes a member of the kinesin light chain family. It associates with kinesin heavy chain through an N-terminal domain, and six tetratricopeptide repeat (TPR) motifs are thought to be involved in binding of cargos such as vesicles, mitochondria, and the Golgi complex. Thus, kinesin light chains function as adapter molecules and not motors per se. Although previously named "kinesin 2", this gene is not a member of the kinesin-2 / kinesin heavy chain subfamily of kinesin motor proteins. Extensive alternative splicing produces isoforms with different C-termini that are proposed to bind to different cargos; however, the full-length nature and/or biological validity of most of these variants have not been determined. [provided by RefSeq]

Alternate Names

hKLC1B, hKLC1G, hKLC1J, hKLC1S, kinesin 2, kinesin 2 60/70kDa, kinesin light chain 1, KLC 1, KLChKLC1N, KNS2AhKLC1P, KNS2hKLC1R, medulloblastoma antigen MU-MB-2.50, MGC15245

Gene Symbol

KLC1

Additional KLC1 Products

Product Documents for KLC1 Antibody (5C3P5)

Certificate of Analysis

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Product Specific Notices for KLC1 Antibody (5C3P5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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