KLF5 Antibody (HL2269) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25544

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2269 expressed in HEK293

Format

Azide and BSA Free
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Product Specifications

Immunogen

Recombinant fragment of human KLF5.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for KLF5 Antibody (HL2269) - Azide and BSA Free

KLF5 Antibody (HL2269) - Azide and BSA Free

Western Blot: KLF5 Antibody (HL2269) - Azide and BSA Free [NBP3-25544] -

Western Blot: KLF5 Antibody (HL2269) - Azide and BSA Free [NBP3-25544] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody [HL2269] (NBP3-25544) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
KLF5 Antibody (HL2269) - Azide and BSA Free

Western Blot: KLF5 Antibody (HL2269) - Azide and BSA Free [NBP3-25544] -

Western Blot: KLF5 Antibody (HL2269) - Azide and BSA Free [NBP3-25544] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody [HL2269] (NBP3-25544) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
KLF5 Antibody (HL2269) - Azide and BSA Free

Western Blot: KLF5 Antibody (HL2269) - Azide and BSA Free [NBP3-25544] -

Western Blot: KLF5 Antibody (HL2269) - Azide and BSA Free [NBP3-25544] - Wild-type (WT) and KLF5 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody [HL2269] (NBP3-25544) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
KLF5 Antibody (HL2269) - Azide and BSA Free

Western Blot: KLF5 Antibody (HL2269) - Azide and BSA Free [NBP3-25544] -

Western Blot: KLF5 Antibody (HL2269) - Azide and BSA Free [NBP3-25544] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody [HL2269] (NBP3-25544) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
KLF5 Antibody (HL2269) - Azide and BSA Free

Western Blot: KLF5 Antibody (HL2269) - Azide and BSA Free [NBP3-25544] -

Western Blot: KLF5 Antibody (HL2269) - Azide and BSA Free [NBP3-25544] - Whole cell extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody [HL2269] (NBP3-25544) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
KLF5 Antibody (HL2269) - Azide and BSA Free

Immunohistochemistry-Paraffin: KLF5 Antibody (HL2269) - Azide and BSA Free [NBP3-25544] -

Immunohistochemistry-Paraffin: KLF5 Antibody (HL2269) - Azide and BSA Free [NBP3-25544] - KLF5 antibody [HL2269] detects KLF5 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. KLF5 stained by KLF5 antibody [HL2269] (NBP3-25544) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
KLF5 Antibody (HL2269) - Azide and BSA Free

Immunohistochemistry-Paraffin: KLF5 Antibody (HL2269) - Azide and BSA Free [NBP3-25544] -

Immunohistochemistry-Paraffin: KLF5 Antibody (HL2269) - Azide and BSA Free [NBP3-25544] - KLF5 antibody [HL2269] detects KLF5 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. KLF5 stained by KLF5 antibody [HL2269] (NBP3-25544) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for KLF5 Antibody (HL2269) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: KLF5

KLF5 encodes a member of the Kruppel-like factor subfamily of zinc finger proteins. Since the protein localizes to the nucleus and binds the epidermal growth factor response element, it is thought to be a transcription factor.

Long Name

Kruppel-Like Factor 5

Alternate Names

BTEB2

Gene Symbol

KLF5

Additional KLF5 Products

Product Documents for KLF5 Antibody (HL2269) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for KLF5 Antibody (HL2269) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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