KMT2A/MLL Antibody - BSA Free

Novus Biologicals | Catalog # NB600-256

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Flow Cytometry, Simple Western, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all KMT2A/MLL Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 2725 and 2775 of human myeloid/lymphoid or mixed-lineage leukemia 1 using the number given in Swiss-Prot entry Q03164 (GeneID 4297).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for KMT2A/MLL Antibody - BSA Free

Western Blot: KMT2A/MLL Antibody [NB600-256]

Western Blot: KMT2A/MLL Antibody [NB600-256]

Western Blot: KMT2A/MLL Antibody [NB600-256] - Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MLL1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Flow Cytometry: KMT2A/MLL Antibody [NB600-256]

Flow Cytometry: KMT2A/MLL Antibody [NB600-256]

Flow Cytometry: KMT2A/MLL Antibody [NB600-256] - Flow cytometry testing of KMT2A/MLL antibody on murine WT and Mll1-/- CD4 T cells that were stimulated in-vitro with anti-CD3/ -CD28 antibodies. Image submitted by our verified customer (see Review section for additional details).
Chromatin Immunoprecipitation: KMT2A/MLL Antibody [NB600-256] - A. 10 ug of was used to immunoprecipitate chromatin from K562 cells according to Ren et al (Genes Dev. 2002 16: 245-256).
Immunoprecipitation: KMT2A/MLL Antibody [NB600-256]

Immunoprecipitation: KMT2A/MLL Antibody [NB600-256]

Immunoprecipitation: KMT2A/MLL Antibody [NB600-256] - Detection of human MLL1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MLL1 antibody NB600-256 used for IP at 3 ug per reaction. MLL1 was also immunoprecipitated by another rabbit anti-MLL1 antibody. For blotting immunoprecipitated MLL1, NB600-256 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
KMT2A/MLL Antibody - BSA Free

Western Blot: KMT2A/MLL Antibody - BSA Free [NB600-256] -

Inhibition of MLL1 promotes the invasion and migration of HTR-8/SVneo cells and represses ferroptosis. HTR-8/SVneo cells were transfected with sh-MLL1, with sh-NC as the control. A: Detection of transfection efficiency by qRT-PCR; then HTR-8/SVneo cells were treated with 10 umol/L Erastin for 24 h to establish a cell model. B: Detection of MLL1 expression in cells by qRT-PCR. C: Detection of cell viability by CCK-8 assay. D-F: Detection of MDA, Fe2+, and GSH levels in cells by kits. G: Detection of ROS levels by fluorescence. H: Detection of MLL1, ASCL4, GPX4, and FTH1 expressions in cells by Western blot. I: Detection of cell invasion and migration by Transwell. The cell experiments were repeated three times independently. Data in panel A were analyzed by t test. Data in panels BCDEFGI were analyzed by one-way ANOVA, and data in panel H were analyzed by two-way ANOVA, followed by Tukey’s multiple comparisons test. **P < 0.01 Image collected and cropped by CiteAb from the following open publication (https://biologydirect.biomedcentral.com/articles/10.1186/s13062-024-005…), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for KMT2A/MLL Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1-5 ug/ml

Immunoprecipitation

2-5 ug/mg of lysate

Simple Western

1:10

Western Blot

1:2000-1:10000
Application Notes
KMT2A/MLL antibody validated for FLOW from a verified customer review.

Reviewed Applications

Read 2 reviews rated 4 using NB600-256 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: KMT2A/MLL

A partial tandem duplication within the MLL-gene (MLL-PTD) can be found in 8% of all patients with karyotypically normal acute myeloid leukemia (AML). MLL possibly acts as a transcriptional regulatory factor.

Alternate Names

ALL1, ALL-1MLL/GAS7, CXXC7TET1-MLL, CXXC-type zinc finger protein 7, EC 2.1.1.43, HRXFLJ11783, HTRX, HTRX1MLL-AF4 der(11) fusion protein, KMT2ACDK6/MLL fusion protein, Lysine N-methyltransferase 2A, MLL/GAS7 fusion protein, MLL/GMPS fusion protein, MLL1, MLL1A, myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog), myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila), Trithorax-like protein, TRX1histone-lysine N-methyltransferase MLL, Zinc finger protein HRX

Entrez Gene IDs

4297 (Human)

Gene Symbol

KMT2A

UniProt

Q03164 (Human)

Additional KMT2A/MLL Products

Product Documents for KMT2A/MLL Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for KMT2A/MLL Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for KMT2A/MLL Antibody - BSA Free

Customer Reviews for KMT2A/MLL Antibody - BSA Free (2)

4 out of 5
2 Customer Ratings
5 Stars
0%
4 Stars
100%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • Name: Matthew Schaller
    Application: Flow Cytometry
    Sample Tested: CD4 T cells cultured in vitro, stimulated with anti-CD3/anti-CD28
    Species: Mouse
    Verified Customer | Posted 08/27/2015
    Flow cytometry for Mll1 on wild type and Mll1-/- CD4 T cells
    KMT2A/MLL Antibody - BSA Free NB600-256
  • Name: Ranveer Singh
    Application: Western Blot
    Sample Tested: Whole cell lysates from HL-60 and HEK 293T cells
    Species: Human
    Verified Customer | Posted 05/07/2014
    Immunoblot with whole cell lysates from HL-60 and HEK 293T cells
    KMT2A/MLL Antibody - BSA Free NB600-256

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