KSHV ORF26 Antibody (2F6B8)

Western Blot: KSHV ORF26 Antibody (2F6B8)BSA Free [NBP1-47357]

Western Blot: KSHV ORF26 Antibody (2F6B8) [NBP1-47357] - Analysis using KSHV ORF26 mouse mAb against TPA induced BCBL-1 cell lysate.

Western Blot: KSHV ORF26 Antibody (2F6B8) [NBP1-47357] -

KSHV lacking SOX produces fewer infectious virions. (A) Western blot of whole cell lysates from iSLK cells containing a KSHV mutant that does not express SOX (SOX stop) or a mutant rescue version of KSHV where SOX expression is restored (SOX stop MR). Lysates were harvested 72 hours post lytic reactivation and blotted with an anti-SOX antibody. Vinculin serves as a loading control. (B, C) RT-qPCR was used to measure the RNA levels of several host (B) or viral (C) transcripts at 48 hours post-reactivation in iSLK cell lines containing the indicated WT or mutant KSHV. Transcript levels were normalized to 18S and to the WT iSLK unreactivated population for (B) for the WT iSLK reactivated population (C). A minimum of three biological replicates are shown. The peak timing of each of the viral genes’ expression is indicated below their name (57). (D) Western blot of lysates from the indicated iSLK cell lines that were either unreactivated or reactivated for 48 hours showing expression of late viral proteins ORF26 and K8.1. Vinculin is used as a loading control. (E) RT-qPCR was used as in (C) to measure the levels of the indicated viral transcripts, but in the presence of 500 mM of the viral DNA replication inhibitor phosphonoacetic acid (PAA). *P ≤ 0.05, one-way ANOVA followed by Dunnett's multiple comparisons test versus WT iSLK. (F) Infectious virion production was measured by flow cytometry on 1 × 106 293T cells infected with 1 ml of supernatant from 1 × 106 reactivated iSLKs. Bars represent the percentage of naïve 293T cells that became GFP positive after 24 hours. Data are from a minimum of four independent biological replicates. ****P ≤ 0.001, one-sample t test versus hypothetical value of 1. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36537232), licensed under a CC-BY license. Not internally tested by Novus Biologicals.