L-(-)-threo-3-Hydroxyaspartic acid

Key Product Details

Product Description

L-(-)-threo-3-Hydroxyaspartic acid is a potent, competitive, transportable EAAT1-4 inhibitor/non-transportable EAAT5 inhibitor. In [³H]-d-Asp uptake assays in HEK293 cells expressing human EAAT1, EAAT2 and EAAT3, K_i values are 11, 19, and 14 μM, respectively. In a FLIPR Membrane Potential (FMP) assay, K_m values are 3.6, 3.8 and 3.2 μM for human EAAT1, EAAT2 and EAAT3, respectively.

Product Specifications for L-(-)-threo-3-Hydroxyaspartic acid

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Preparing Stock Solutions for L-(-)-threo-3-Hydroxyaspartic acid

The following data is based on the product molecular weight 149.10.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

14 13 12 11 10 9 8 7 Recalculate

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	6.71 mL	33.53 mL	67.07 mL
5 mM	1.34 mL	6.71 mL	13.41 mL
10 mM	0.67 mL	3.35 mL	6.71 mL
50 mM	0.13 mL	0.67 mL	1.34 mL

Calculators

Background References

References are publications that support the biological activity of the product. See our Citations tab to view 11 publications citing the usage of this product.

• Jensen and Bräuner-Osborne Pharmacological characterization of human excitatory amino acid transporters EAAT1, EAAT2 and EAAT3 in a fluorescence-based membrane potential assay. Biochem.Pharmacol. 2004 PMID: 15135308
• McBean and Roberts Neurotoxicity of L-glutamate and DL-threo-3-hydroxyaspartate in the rat striatum. J.Neurochem. 1985 PMID: 2856883
• Nakamura (2S,3S,4R)-2-(carboxycyclopropyl)glycine, a potent and competitive inhibitor of both glial and neuronal uptake of glutamate. Neuropharmacology 1993 PMID: 7901789
• Johnston Potentiation of L-glutamate and L-aspartate excitation of cat spinal neurones by the stereoisomers of threo-3-hydroxyaspartate. J.Neurochem. 1980 PMID: 7452241

Product Documents for L-(-)-threo-3-Hydroxyaspartic acid