Human Lactate Dehydrogenase A/LDHA ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-39736

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, tissue homogenates and other biological fluids (100 uL)

Sensitivity

0.32 ng/mL (example only; lot dependent)

Assay Range

0.781 - 50 ng/mL (example only; lot dependent)
View all Lactate Dehydrogenase A/LDHA ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Description

Assay Length: 4.5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV 12 (example only; lot dependent)

Scientific Data Images for Human Lactate Dehydrogenase A/LDHA ELISA Kit (Colorimetric)

Human Lactate Dehydrogenase A/LDHA ELISA Kit (Colorimetric)

ELISA: Human Lactate Dehydrogenase A/LDHA ELISA Kit (Colorimetric) [NBP3-39736]

ELISA: Human Lactate Dehydrogenase A/LDHA ELISA Kit (Colorimetric) [NBP3-39736] - Standard Curve Reference

Kit Contents for Human Lactate Dehydrogenase A/LDHA ELISA Kit (Colorimetric)

  • Detection Reagent A
  • Detection Reagent B
  • Diluent Buffer
  • Instruction manual
  • Plate sealer for 96 wells
  • Pre-coated 96T strip plate
  • Standard
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Lactate Dehydrogenase A/LDHA

Lactate dehydrogenase (LDH) is an ubiquitous enzyme commonly found in wide variety of organisms, including plants and microbes. LDH is involved in the interconversion of the pyruvate and NADH to lactate and NAD+. It is also called Hydroxybutyrate Dehydrogenase (HBD), due to the fact that it can catalyze the oxidation of hydroxybutyrate (1). In mammals, three types of LDH subunits (35 kDa) are encoded by the genes Ldh-A, Ldh-B, and Ldh-C, forming various terameric isoenzymes (140 kDa). Lactate dehydrogenase A (LDH-A, muscle subunit, LDH-M) is involved in the final step of anaerobic glycoysis and catalyzes the conversion of L-lactate and NAD to pryruvate and NADH. While it is predominantly expressed in muscle tissue, it is hormonally regulated in rodents and overexpressed during mammary gland tumorigenesis (2). A mutation that cause deficiency in LDH-A has been implicated in exertional myoglobinuria (3).

Alternate Names

LDH1, LDHA, LDHM

Gene Symbol

LDHA

Additional Lactate Dehydrogenase A/LDHA Products

Product Documents for Human Lactate Dehydrogenase A/LDHA ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human Lactate Dehydrogenase A/LDHA ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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